Cultured rat cortical astrocytes synthesize melatonin: absence of a diurnal rhythm
Cultured rat cortical astrocytes synthesize melatonin: absence of a diurnal rhythm
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培养的大鼠皮质星形胶质细胞合成褪黑激素：缺乏昼夜节律
DOI：
10.1111/j.1600-079x.2007.00466.x
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发表时间：
2007-10-01
影响因子：
10.3
通讯作者：
Zhou, Jiang-Ning
中科院分区：
文献类型：
--
作者：
Liu, Ya-Jing;Zhuang, Jun;Zhou, Jiang-Ning
褪黑激素不仅在昼夜节律的调节中起主要作用，而且还参与抗氧化防御和免疫调节。循环褪黑激素水平主要来自松果体，而褪黑激素的其他来源也有报道。在这里，我们第一次表明，从大鼠皮层和胶质瘤C6细胞系的星形胶质细胞在体外合成褪黑激素。此外，我们显示的存在下，5-羟色胺，褪黑激素的前体和褪黑激素合成途径中的两个关键酶，即N-乙酰转移酶和羟吲哚-O-甲基转移酶在培养的大鼠皮层星形胶质细胞。褪黑激素释放到培养基中没有表现出昼夜变化。这表明星形胶质细胞是大鼠大脑中褪黑激素的局部来源。其确切的生理功能仍然是未来研究的主题。
Melatonin not only plays a major role in the regulation of circadian rhythms, but is also involved in antioxidative defense and immunomodulation. Circulating melatonin levels are derived primarily from the pineal gland while other sources of melatonin have also been reported. Here, we show for the first time that astrocytes from the rat cortex and glioma C6 cell line synthesize melatonin in vitro. In addition, we show the presence of serotonin, the precursor of melatonin and the two key enzymes in the pathway of melatonin synthesis, i.e. N-acetyltransferase and hydroxyndole-O-methyltransferase in the cultured rat cortical astrocytes. Release of melatonin into the culture medium showed no diurnal changes. These point to astrocytes as a local source of melatonin in the rat brain. Its exact physiological function remains a topic for future studies.