Gracilibacillus oryzae sp. nov., isolated from rice seeds.
Gracilibacillus oryzae sp. nov., isolated from rice seeds.
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DOI：
10.1099/ijsem.0.004427
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发表时间：
2020-09
影响因子：
2.8
通讯作者：
Shan-Wen He;Xing Wang;He-Bao Guo;Ji-Gang Han;K. Thin;Ju-sheng Gao;Li-An Ma;Xiao-Xia Zhang
中科院分区：
文献类型：
--
作者：
Shan-Wen He;Xing Wang;He-Bao Guo;Ji-Gang Han;K. Thin;Ju-sheng Gao;Li-An Ma;Xiao-Xia Zhang
从表面灭菌的水稻种子中分离到一株革兰氏染色阳性、厌氧兼性、内孢子形成的细菌，命名为菌株TD 8 T。16 S rRNA基因的系统发育分析表明，菌株TD 8 T应归属于纤细芽孢杆菌属（95.2- 99.0%序列相似性）;与解脲纤细芽孢杆菌（Gracilibacillusureilyticus）的同源性最高，为1.7727T新疆纤细芽孢杆菌（Gracilibacillus xinjiangensis）1.12449T（98.9%）和日本龙纤细芽孢杆菌（Gracilibacillus dipsosauri）1.3642T（97.5%）。化学分类学分析表明，甲基萘醌-7（MK-7）是主要的类异戊二烯醌。细胞极性脂主要为二磷脂酰甘油、磷脂酰甘油和一种未鉴定的磷脂，脂肪酸主要为anteiso-C15：0、anteiso-C17：0、iso-C15：0、C16：0和iso-C16：0，支持该菌株属于纤细芽孢杆菌属。菌株TD 8 T与解脲纤细芽孢杆菌（Gracilibacillusureilyticus）CGMCC 1.7727T的DNA-DNA数字杂交值低于70%（22.60%），平均核苷酸同源性为79.54± 5.09%，表明菌株TD 8 T为纤细芽孢杆菌属中的一个新种。基因组DNA G+C含量为37.5%。根据生理生化特性和基因型数据，菌株TD 8 T代表纤细芽孢杆菌属的一个新种，建议将其命名为纤细芽孢杆菌属（Gracilibacillussp. nov.）。模式菌株为TD 8 T（=ACCC 61556 T =CICC 24889 T =JCM 33537 T）。
A Gram-stain-positive, facultatively anaerobic, endospore-forming bacterium, designated strain TD8T, was isolated from surface-sterilized rice seeds (Oryza sativa L.). Phylogenetic analysis of the 16S rRNA gene indicated that strain TD8T should be placed within the genus Gracilibacillus (95.2-99.0 % sequence similarity); it exhibited highest similarities to Gracilibacillus ureilyticus CGMCC 1.7727T (99.0 %), 'Gracilibacillus xinjiangensis' CGMCC 1.12449T (98.9 %) and Gracilibacillus dipsosauri CGMCC 1.3642T (97.5 %). Chemotaxonomic analysis showed that menaquinone-7 (MK-7) was the major isoprenoid quinone. Diphosphatidylglycerol, phosphatidylglycerol and one unidentified phospholipid were the major cellular polar lipids, and the major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0, C16 : 0 and iso-C16 : 0, which supported the allocation of the strain to the genus Gracilibacillus. The digital DNA-DNA hybridization value between strain TD8T and Gracilibacillus ureilyticus CGMCC 1.7727T was lower than 70 % (22.60 %), and the average nucleotide identity score was 79.54±5.09 %, suggesting that strain TD8T represented a novel species in the genus Gracilibacillus. The genomic DNA G+C content was 37.5 %. Based on physiological and biochemical characteristics and genotypic data, strain TD8T represents a novel species of the genus Gracilibacillus, for which the name Gracilibacillus oryzae sp. nov. is proposed. The type strain is TD8T (=ACCC 61556T=CICC 24889T=JCM 33537T).