Partial purification of transcriptionally active nucleosomes from trout testis cells.
Partial purification of transcriptionally active nucleosomes from trout testis cells.
复制标题
从鳟鱼睾丸细胞中部分纯化转录活性核小体。
作者：
B. Levy;G. H. Dixon
富含结构非组蛋白蛋白和转录的DNA序列的单核细胞体（MN1），通过限制性消化鳟鱼睾丸细胞核微球菌核酸酶，然后在0.1 M NaCl中选择性溶解。这些单体由四个内部组蛋白加上化学计量量的HMG组的非组蛋白H6组成，与140个碱基对的DNA复合。杂交实验表明，MN1 DNA富含与细胞质多聚腺苷酸化RNA互补的序列。
Mononucleosomes (MN1) enriched in structural non-histone proteins and transcribed DNA sequences were obtained by limited digestion of trout testis nuclei with micrococcal nuclease followed by selective solubilization in 0.1 M NaCl. These monosomes consist of the four inner histones plus stoichiometric amounts of the non-histone protein H6, of the HMG group, complexed with 140 base pairs of DNA. Hybridization experiments indicate that MN1 DNA is enriched in sequences complementary to cytoplasmic polyadenylated RNA.