Selective ATP recognition by boronic acid-appended cyclodextrin and a fluorescent probe supramolecular complex in water
Selective ATP recognition by boronic acid-appended cyclodextrin and a fluorescent probe supramolecular complex in water
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水中硼酸环糊精和荧光探针超分子复合物选择性识别 ATP
DOI：
10.1039/d3nj00139c
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发表时间：
2023
影响因子：
3.3
通讯作者：
Hayashita Takashi
中科院分区：
文献类型：
--
作者：
Suzuki Yota;Masuko Masakage;Hashimoto Takeshi;Hayashita Takashi
已有多种基于有机化合物的三磷酸腺苷(ATP)化学传感器的报道，然而，传统的设计在其水溶性、选择性和灵敏度方面存在问题。本论文基于“环糊精(γ)超分子化学传感器”的概念，设计了一种含硼酸的环糊精-环糊精与锌-二吡啶胺基芘探针的超分子络合物(ZN-1/FB-γ-CyD)。作为概念验证，我们已经证明了该配合物在生理pH条件下对水中的三磷酸腺苷表现出良好的选择性和灵敏度，这是因为锌-1/FBγCyd通过协同作用与三磷酸腺苷有效地自组装而得到了强荧光产物。值得注意的是，锌-1/FB-γ-CyD对−显示出极强的亲和力，其条件形成常数为(7.71±0.09)×106M·cYD，检测下限为18.9nM。此外，锌-1/FB-γ细胞色素D可以监测加入apyrase后，ATP浓度的变化，该酶能将ATP分解成一磷酸腺苷和一个磷酸根离子。我们相信，我们提出的基于CyD的超分子化学传感器的方法将提出一种新的设计，在荧光分子识别研究领域实现未知的选择性。
Various organic compound-based chemosensors for adenosine triphosphate (ATP) have been reported; however, the conventional designs have problems with their water solubility, selectivity, and sensitivity. Herein, a supramolecular complex of boronic acid-appended γ-cyclodextrin and zinc-dipicolylamine-based pyrene probe (Zn-1/FBγCyD) was designed based on the concept of a “cyclodextrin (CyD)-based supramolecular chemosensor”. As a proof-of-concept, we have demonstrated that this complex exhibits a turn-on fluorescence response to ATP in water at physiological pH with excellent selectivity and sensitivity, owing to the efficient self-assembly of Zn-1/FBγCyD with ATP through synergistic interactions to afford a strongly fluorescent product. Remarkably, Zn-1/FBγCyD demonstrated extremely strong affinity for ATP with the conditional formation constant of (7.71 ± 0.09) × 106 M−1, resulting in the low limit of detection of 18.9 nM. Furthermore, Zn-1/FBγCyD can monitor changes in ATP concentration after the addition of apyrase, hydrolysing ATP to adenosine monophosphate and a phosphate ion. We believe that the presented approach of a CyD-based supramolecular chemosensor will propose a novel design that realises unexplored selectivity in the research field of fluorescent molecular recognition.