Differential Gene Expression Profiling of Oesophageal Squamous Cell Carcinoma by DNA Microarray and Bioinformatics Analysis
Differential Gene Expression Profiling of Oesophageal Squamous Cell Carcinoma by DNA Microarray and Bioinformatics Analysis
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DOI：
10.1177/147323001003800603
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发表时间：
2010-11-01
影响因子：
1.6
通讯作者：
Li, J. S.
中科院分区：
文献类型：
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作者：
Li, J. D.;Feng, Q. C.;Li, J. S.
对食道鳞状细胞癌(ESCC)患者的原发肿瘤组织和癌旁非肿瘤组织进行差异基因表达谱分析。用全基因组芯片分析了从4例ESCC患者的食管鳞癌组织和匹配的正常食道上皮中提取的RNA。还进行了生物信息学分析，以确定哪些基因和途径可能在ESCC的发生发展中起重要作用。共鉴定出570个差异表达基因：303个基因在食管鳞癌组织中表达上调，267个基因在食管鳞癌组织中表达下调。基因本体论分析表明，这些基因的主要分子功能与胞外区、胶原和内肽酶抑制物活性有关。通路分析揭示了与差异表达谱高度相关的七条通路或网络。基因组分析表明，POD1KOUP基因组显著丰富，包含15个匹配基因。因此，大量基因参与食管癌的发生发展，并参与多种细胞过程和途径。
Differential gene expression profiling was carried out on primary tumour tissues and adjacent non-neoplastic tissues from patients with oesophageal squamous cell carcinoma (ESCC). RNA extracted from ESCC tissues and matched normal oesophageal epithelium of four ESCC patients was analysed using whole-genome microarrays. Bioinformatics analysis was also carried out to ascertain which genes and pathways may be important in the carcinogenesis of ESCC. A total of 570 genes were identified that differed significantly in expression: 303 genes were up-regulated and 267 genes were down-regulated in ESCC tissues compared with normal oesophageal epithelium. Gene ontology analysis showed that the primary molecular functions of these genes were related to the extracellular region, collagen and endopeptidase inhibitor activity. Pathway analysis revealed seven pathways or networks highly associated with the differential expression profile. Gene set analysis showed that the POD1_KO_UP gene set was significantly enriched, containing 15 matching genes. Thus, a large number of genes are involved in the carcinogenesis of ESCC and participate in various cell processes and pathways.