Genetic functions promoting homologous recombination in Escherichia coli: a study of inversions in phage lambda.
Genetic functions promoting homologous recombination in Escherichia coli: a study of inversions in phage lambda.
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促进大肠杆菌同源重组的遗传功能：噬菌体 lambda 反转的研究。
DOI：
10.1093/genetics/115.1.11
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发表时间：
1987
期刊：
影响因子：
3.3
通讯作者：
Smith,GR
中科院分区：
文献类型：
--
作者：
Ennis,DG;Amundsen,SK;Smith,GR
我们研究了λ噬菌体衍生物中的同源重组，该衍生物含有两个反向的1.4kb重复序列。插入的2.5 kb片段的倒位通过大肠杆菌RecBC途径有效地发生，但通过λ Red途径或E. coliRecE或RecF途径。由RecBCD途径的反转刺激Chi网站位于可逆段的右侧，这种刺激指数下降约2倍，为每2.2 kb之间的可逆段和Chi网站。除了RecA蛋白和RecBCD酶之外，RecBC途径的反转还需要单链DNA结合蛋白、DNA促旋酶、DNA聚合酶I和DNA连接酶。反转似乎发生在分子内或分子间。这些结果进行了讨论的框架内的RecBC途径的同源重组的当前分子模型。
We have studied homologous recombination in a derivative of phage λ containing two 1.4-kb repeats in inverted orientation. Inversion of the intervening 2.5-kb segment occurred efficiently by theEscherichia coliRecBC pathway but markedly less efficiently by the λ Red pathway or theE. coliRecE or RecF pathways. Inversion by the RecBCD pathway was stimulated by Chi sites located to the right of the invertible segment; this stimulation decreased exponentially by a factor of about 2 for each 2.2 kb between the invertible segment and the Chi site. In addition to RecA protein and RecBCD enzyme, inversion by the RecBC pathway required single-stranded DNA binding protein, DNA gyrase, DNA polymerase I and DNA ligase. Inversion appeared to occur either intra- or intermolecularly. These results are discussed in the framework of a current molecular model for the RecBC pathway of homologous recombination.