Proteome analysis of primary neurons and astrocytes from rat cerebellum
Proteome analysis of primary neurons and astrocytes from rat cerebellum
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DOI：
10.1021/pr049774v
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发表时间：
2005-05-01
影响因子：
4.4
通讯作者：
Lubec, G
中科院分区：
文献类型：
--
作者：
Yang, JW;Rodrigo, R;Lubec, G
神经元和星形胶质细胞是大脑中的主要细胞类型，具有独特的形态和功能特征。尽管对大脑进行了一些蛋白质组学研究，但对单个脑细胞的研究仍然有限。然而，必须生成神经元和星形胶质细胞的单个蛋白质组，才能将蛋白质表达分配给细胞类型而不是组织。我们的目的是使用二维凝胶电泳和随后的凝胶内消化以及 MALDI-TOF/TOF 提供大鼠原代神经元和星形胶质细胞的图谱。明确鉴定了对应于神经元中 226 个单独蛋白质的 428 个蛋白质点和代表星形胶质细胞中 228 个蛋白质的 406 个蛋白质点。蛋白质组数据包括来自神经元和星形胶质细胞中差异表达的多个级联的蛋白质，并证明了抗氧化剂、信号传导、分子伴侣、细胞骨架、核酸结合、蛋白酶体和代谢蛋白质的特定表达模式。我们在此提出了原代大鼠原代神经元和星形胶质细胞蛋白质组的参考数据库，并为这些结构提供了分析工具。给出了几种蛋白质类别的伴随表达模式，并提出了潜在的神经元和星形细胞标记候选物。
Neurons and astrocytes are predominant cell types in brain and have distinguished morphological and functional features. Although several proteomics studies were carried out on the brain, work on individual brain cells is limited. Generating individual proteomes of neurons and astrocytes, however, is mandatory to assign protein expression to cell types rather than to tissues. We aimed to provide maps of rat primary neurons and astrocytes using two-dimensional gel electrophoresis with subsequent in-gel digestion, followed by MALDI-TOF/TOF. 428 protein spots corresponding to 226 individual proteins in neurons and 406 protein spots representing 228 proteins in astrocytes were unambiguously identified. Proteome data include proteins from several cascades differentially expressed in neurons and astrocytes, and specific expressional patterns of antioxidant, signaling, chaperone, cytoskeleton, nucleic acid binding, proteasomal, and metabolic proteins are demonstrated. We herein present a reference database of primary rat primary neuron and astrocyte proteomes and provide an analytical tool for these structures. The concomitant expressional patterns of several protein classes are given and potential neuronal and astrocytic marker candidates are presented.