Effects of electroacupuncture on retinal nerve growth factor and brain-derived neurotrophic factor expression in a rat model of retinitis pigmentosa
Effects of electroacupuncture on retinal nerve growth factor and brain-derived neurotrophic factor expression in a rat model of retinitis pigmentosa
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DOI：
10.1016/j.brainres.2006.03.074
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发表时间：
2006-05-30
期刊：
影响因子：
2.9
通讯作者：
Aloe, Luigi
中科院分区：
文献类型：
--
作者：
Pagani, Lucia;Manni, Luigi;Aloe, Luigi
本研究的目的是调查电针（EA）对遗传性色素性视网膜炎（IRP）大鼠视网膜变性进展的影响，并将该事件与神经营养素的视网膜表达相关联。 30 天大的皇家外科学院 (RCS) 大鼠连续 11 天接受每天 25 分钟的低频 EA 治疗。在最后一次 EA 治疗后 1 小时处死对照未治疗和 EA 治疗的大鼠，并取出其视网膜进行生化、分子和免疫组织化学分析。我们的数据显示，在视网膜细胞变性的关键发育阶段，每天对 RCS 大鼠进行低频 EA 治疗 11 天，会导致视网膜神经生长因子 (NGF) 和 NGF 高亲和力受体 (TrkA) 表达增加；外核层（ONL）厚度增加；并增强血管化。这些发现表明，电针治疗对 RCS 大鼠 IRP 样视网膜变性的发展可能具有有益作用，以及电针可能通过调节视网膜细胞中 NGF 和脑源性神经营养因子 (BDNF) 和/或其受体发挥其作用的机制。 (c) 2006 Elsevier B.V. 保留所有权利。
The aim of this study was to investigate the effect of electroacupuncture (EA) on the progression of retinal degeneration in rats affected by inherited retinitis pigmentosa (IRP) and to correlate this event with the retinal expression of neurotrophins. Thirty-day-old Royal College of Surgeons (RCS) rats were exposed to 25-min-long daily sessions of low-frequency EA for 11 consecutive days. Control-untreated and EA-treated rats were sacrificed 1 h after the last EA session, and their retina removed for biochemical, molecular, and immunohistochemical analyses. Our data revealed that daily sessions of low-frequency EA for 11 days to RCS rats during a critical developmental stage of retinal cell degeneration cause an increase of retinal nerve growth factor (NGF) and NGF high-affinity receptor (TrkA) expression; and increase of outer nuclear layer (ONL) thickness; and enhanced vascularization. These findings suggest the possible beneficial effects of EA treatment in the development of IRP-like retinal degeneration of RCS rats and that the mechanism through which EA might exerts its action on the regulation of NGF and brain-derived neurotrophic factor (BDNF) and/or their receptors in retinal cells. (c) 2006 Elsevier B.V. All rights reserved.