Establishment of a novel equine cell line for isolation and propagation of equine herpesviruses
Establishment of a novel equine cell line for isolation and propagation of equine herpesviruses
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DOI：
10.1292/jvms.69.989
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发表时间：
2007-09-01
影响因子：
1.2
通讯作者：
Matsumura, Tornio
中科院分区：
文献类型：
--
作者：
Maeda, Ken;Yasumoto, Shigeru;Matsumura, Tornio
本研究用编码类人猿病毒40 （SV40）大T抗原的表达质粒转染原代胎马肾（FHK）细胞，建立了马源性细胞系。克隆的细胞系名为FHK-Tcl3，生长良好，通过1:3分裂可以繁殖30倍以上。马疱疹病毒(EHV)-1和EHV-4在FHK-Tcl3中复制良好。利用FHK-Tcl3从野外采集的马标本中分离到EHV-2和EHV-4， EHV-3也在FHK-Tcl3中繁殖。这些结果表明，该新细胞株FHK-Tcl3可用于马疱疹病毒的分离和繁殖。
In the present study, an equine-derived cell line was established by transfecting primary fetal horse kidney (FHK) cells with expression plasmid encoding simian virus 40 (SV40) large T antigen and then cloning them by limiting dilution. The cloned cell line, named FHK-Tcl3, grew well and could be propagated over 30 times by splitting them 1:3. Equine herpesvirus (EHV)-1 and EHV-4 replicated well in FHK-Tcl3. EHV-2 and EHV-4 were isolated from samples collected from horses in the field using FHK-Tcl3, and EHV-3 also propagated in FHK-Tcl3. These results indicated that this novel cell line, FHK-Tcl3, can be used for isolation and propagation of equine herpesviruses.