The purification and characterization of rat placental lactogen.
The purification and characterization of rat placental lactogen.
复制标题
大鼠胎盘催乳素的纯化及表征。
DOI：
10.1210/endo-97-3-621
复制
发表时间：
1975
期刊：
影响因子：
4.8
通讯作者：
H. G. Friesen
中科院分区：
文献类型：
--
作者：
May C. Robertson;H. G. Friesen
本文报道了一种用特异性放射受体分析法（RRA）纯化大鼠胎盘催乳素的方法。方法包括硫酸铵沉淀、Sephadex G-100凝胶过滤、DEAE-纤维素和CM-Sephadex离子交换层析和制备等电聚焦。分离预处理的结果在1300倍的纯化和10%的产率的大鼠胎盘催乳素。通过RRA进行的效价估计表明，纯化激素的活性为绵羊催乳素标准品（25 IU/mg）的41%，但活性为NIH人胎盘催乳素制剂的169%。大鼠胎盘催乳素的聚丙烯酰胺凝胶电泳（pH8.9）和等电聚焦分析显示，RRA中有2种主要成分和2种次要成分。
A method has been developed for the purification of rat placental lactogen employing a specific radioreceptor assay (RRA). The method involves precipitation with ammonium sulfate, gel filtration on Sephadex G-100, ion-exchange chromatography on DEAE-cellulose and CM-Sephadex, and preparative isoelectric focusing. The isolation precedure results in a 1300-fold purification and a 10% yield of rat placental lactogen. Potency estimates by RRA indicate that the purified hormone is 41% as active as the ovine prolactin standard (25 I U/mg), but 169% as active as the NIH human placental lactogen preparation. Polyacrylamide gel electrophoresis at pH 8.9 annd analytical isoelectric focusing of the rat placental lactogen reveal 2 major and 2 minor components, all of which are active in the RRA.