Surfactants as fungal parasite control agents in oleaginous microalga, Graesiella sp. WBG-1, mass culture
Surfactants as fungal parasite control agents in oleaginous microalga, Graesiella sp. WBG-1, mass culture
复制标题
表面活性剂作为产油微藻 Graesiella sp 中的真菌寄生虫控制剂。
DOI：
10.1016/j.algal.2019.101539
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发表时间：
2019-08-01
影响因子：
5.1
通讯作者：
Li, Yeguang
中科院分区：
文献类型：
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作者：
Ding, Yi;Wen, Xiaobin;Li, Yeguang
微生物污染是油脂微藻大量培养成功的一个毁灭性威胁。真菌寄生虫很可能感染培养的微藻，使藻类生物量大大减少到极低的水平。在本研究中，四种表面活性剂被用来控制真菌寄生虫的培养的含油微生物，Graesiella sp. WBG-1，首次。在实验室规模的实验中，10-70毫克L-1的十二烷基苯磺酸钠（SDBS）和椰子二乙醇酰胺（CDEA）完全防止真菌寄生虫感染。此外，SDBS对Graesiella sp. WBG-1的最终生物量、脂质含量和脂肪酸谱没有不利影响。此外，中试规模和工业规模的实验清楚地证实，10毫克L-1 SDBS有效地防止真菌寄生虫感染，而不会产生负面影响的生物量和脂质含量。Graesiella sp. WBG-1在200 m2和1000 m2的开放式跑道池塘中的油脂含量均达到30%以上。这里展示的实用SDBS方法可以廉价地应用于中试规模（40，000 L反应器为0.7美元）和工业规模（200，000 L反应器为3.6美元）。因此，SDBS可能是工业规模培养Graesiella sp. WBG-1和可能的其他产油微生物中理想的真菌寄生虫控制剂。这种有效且低成本的方法推进了利用产油微藻作为经济可行的生物柴油生产策略的前景。
Microbial contamination is a devastating threat to the success of oleaginous microalgae mass cultivation. Fungal parasites are likely to infect cultivated microalgae, greatly reducing algal biomass to extremely low levels. In the present study, four surfactants were used to control fungal parasites in the culture of the oleaginous microalga, Graesiella sp. WBG-1, for the first time. In lab-scale experiments, 10-70 mg L-1 sodium dodecylbenzene sulfonate (SDBS) and coconut diethanolamide (CDEA) completely prevented fungal parasite infection. Moreover, SDBS had no adverse influence on the final biomass, lipid contents, and fatty acid profiles of Graesiella sp. WBG-1. Additionally, pilot-scale and industrial-scale experiments clearly confirmed that 10 mg L-1 SDBS effectively prevented fungal parasite infections without negatively affecting biomass and lipid contents. Furthermore, the lipid contents of Graesiella sp. WBG-1 reached > 30%, both in 200 m(2) and 1000 m(2) open raceway ponds. The practical SDBS approach demonstrated here can be inexpensively applied on pilot- (0.7 USD for a 40,000 L reactor) and industrial- (3.6 USD for a 200,000 L reactor) scales. Therefore, SDBS may be a desirable fungal parasite control agent in industrial scale cultivation of Graesiella sp. WBG-1 and, possibly, other oleaginous microalga. This effective and low cost approach advanced the prospect of using oleaginous microalgae as an economically viable strategy for the biodiesel production.