Using proteomic analysis of the human amniotic fluid to identify histologic chorioamnionitis
Using proteomic analysis of the human amniotic fluid to identify histologic chorioamnionitis
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DOI：
10.1097/aog.0b013e31816102aa
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发表时间：
2008-02-01
影响因子：
7.2
通讯作者：
Buhimschi, Catalin S.
中科院分区：
文献类型：
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作者：
Buhimschi, Irina A.;Zambrano, Eduardo;Buhimschi, Catalin S.
目的：评估组织学绒毛膜炎和四种羊水蛋白质组学炎症标志物（防御素2和1，钙粒蛋白C和A）之间的关系：158例单胎妊娠妇女进行了临床指征性羊膜穿刺术，以排除早产或早产胎膜早破背景下的炎症和感染。使用表面增强激光解吸电离飞行时间质谱法从羊水中产生蛋白质组指纹（质量限制评分）。Mass Restricted评分范围为0至4（无至所有四种生物标志物存在），与羊膜内炎症的严重程度直接相关。分析生物标志物的存在或不存在与胎盘病理学的关系。组织学绒毛膜炎的严重程度标准为3个阶段和4级炎症的羊膜，绒毛膜蜕膜和chorionicplate.Results：组织学绒毛膜炎的患病率为64%（I期12 /0，II期16%，III期37%）。Mass Restricted评分与组织学分期显著相关（r=0.539，P
OBJECTIVE: To estimate the relationship between histologic chorioamnionitis and four amniotic fluid proteomic biomarkers characteristic of inflammation (defensins 2 and 1, calgranulins C and A).METHODS: One hundred fifty-eight women with singleton pregnancies had a clinically indicated amniocentesis to rule out inflammation and infection in the context of preterm labor or preterm premature rupture of membranes. A proteomic fingerprint (Mass Restricted score) was generated from amniotic fluid using surface-enhanced laser desorption ionization time-of-flight mass spectrometry. The Mass Restricted score ranges from 0 to 4 (none to all four biomarkers present) in direct relationship with severity of intra-amniotic inflammation. Presence or absence of biomarkers was analyzed in relationship to placental pathology. Criteria for severity of histologic chorioamnionitis were 3 stages and 4 grades of inflammation of the amnion, choriodecidua and chorionic plate.RESULTS: The prevalence of histologic chorioamnionitis was 64% (stage I 12 /0, stage II 16%, and stage III 37%). The Mass Restricted score significantly correlated with stages of histologic chorioamnionitis (r=0.539, P