Chlamydia pneumoniae infection of brain cells:: An in vitro study
Chlamydia pneumoniae infection of brain cells:: An in vitro study
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DOI：
10.1016/j.neurobiolaging.2006.02.014
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发表时间：
2007-04-01
影响因子：
4.2
通讯作者：
Stassen, Frank R. M.
中科院分区：
文献类型：
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作者：
Boelen, Ellen;Steinbusch, Harry W. M.;Stassen, Frank R. M.
受神经系统疾病和感染之间相关性的启发，我们确定了脑细胞对肺炎衣原体（Cpn）的易感性。小鼠星形胶质细胞（C8 D1 A）、神经元（NB 41 A3）和小胶质细胞（BV-2）细胞系接种Cpn。在不同时间点通过免疫荧光和实时PCR建立感染。通过将感染细胞的培养基转移到检测层来评估生产性感染。最后检测感染后细胞凋亡和坏死情况。我们的数据表明，神经元细胞系是高度敏感的Cpn，产生可行的后代，并易于死亡后感染坏死。除了细胞外Cpn的产量较高和坏死不太明显外，星形胶质细胞细胞系中的Cpn嗜性是相似的。相比之下，小胶质细胞系对Cpn具有高度抗性，因为免疫组织化学迹象在24小时后几乎完全消失。然而，可以检测到显着的Cpn DNA量，表明Cpn持久性。观察到低存活后代和几乎没有任何坏死的小胶质细胞。进一步的研究是必要的，以确定这些细胞类型是否在体内环境中对Cpn显示出相同的敏感性。(c)2006年爱思唯尔公司All rights reserved.
Inspired by the suggested associations between neurological diseases and infections, we determined the susceptibility of brain cells to Chlamydia pneumoniae (Cpn). Murine astrocyte (C8D1A), neuronal (NB41A3) and microglial (BV-2) cell lines were inoculated with Cpn. Infection was established by immunofluorescence and real-time PCR at various time points. Productive infection was assessed by transferring medium of infected cells to a detection layer. Finally, apoptosis and necrosis post-infection was detrmined. Our data demonstrate that the neuronal cell line is highly sensitive to Cpn, produces viable progeny and is prone to die after infection by necrosis. Cpn tropism was similar in an astrocyte cell line, apart from the higher production of extracellular Cpn and less pronounced necrosis. In contrast, the microglial cell line is highly resistant to Cpn as the immunohistochemical signs almost completely disappeared after 24 h. Nevertheless, significant Cpn DNA amounts could be detected, suggesting Cpn persistence. Low viable progeny and hardly any necrotic microglial cells were observed. Further research is warranted to determine whether these cell types show the same sensitivity to Cpn in an in vivo setting. (c) 2006 Elsevier Inc. All rights reserved.