Validation of a high performance liquid chromatography analysis for the determination of noradrenaline and adrenaline in human urine with an on-line sample purification.
Validation of a high performance liquid chromatography analysis for the determination of noradrenaline and adrenaline in human urine with an on-line sample purification.
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DOI：
10.1016/s0039-9140(99)00029-6
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发表时间：
1999-09
期刊：
影响因子：
6.1
通讯作者：
Å. Hansen;J. Kristiansen;J. L. Nielsen;K. Byrialsen;J. M. Christensen
中科院分区：
文献类型：
--
作者：
Å. Hansen;J. Kristiansen;J. L. Nielsen;K. Byrialsen;J. M. Christensen
建立了一种在线净化、高效液相色谱荧光检测法测定人尿中儿茶酚胺的方法。该方法进行了评价，使用样本的合并尿液中掺入的儿茶酚胺和验证的测量健康个体的尿液中的儿茶酚胺在一个领域的研究。实验室方法评价研究表明，去甲肾上腺素和肾上腺素的方法回收率分别为0.82（置信区间（CI）：0.79-0.86）和0.92（CI：0.89-0.95）。因此，采用0.82− 1和0.92− 1的校正因子来校正该系统效应的测量结果。此外，使用国际标准化组织推荐的BIPM方法为分析方法生成不确定度预算。该方法的相对不确定度估计为10- 12%，与方法评价中观察到的相对变异性一致。根据EURACHEM指南文件1关于化学实验室认证的准确度和精密度评价该方法。实地研究表明，该方法的标准偏差很低，足以区分从事两种不同垃圾收集过程的人员，即使用四轮集装箱收集垃圾和使用垃圾箱收集垃圾。
A high performance liquid chromatography (HPLC) method with fluorescence detection including an on-line purification was established for determination of catecholamines in human urine. The method was evaluated using samples of pooled urine spiked with catecholamines and validated for measurements of catecholamines in urine of healthy individuals in a field study. The laboratory method evaluation study showed that the recovery of the method was 0.82 (confidence interval (CI): 0.79–0.86) and 0.92 (CI: 0.89–0.95) for noradrenaline and adrenaline, respectively. Thus, correction factors of 0.82−1and 0.92−1were applied to correct the measurement results for this systematic effect. Furthermore, an uncertainty budget was generated for the analytical method using the BIPM-approach recommended by the International Organization for Standardization. The relative uncertainty of the method was estimated to be 10–12%, which was consistent with the observed relative variability found in the method evaluation. The method was evaluated in accordance with EURACHEM Guidance Document No 1 concerning accreditation for chemical laboratories with respect to accuracy and precision. The field study showed that the standard deviation of the method was sufficiently low to distinguish between persons working with two different processes of garbage collection, i.e. collection using four wheeled containers versus collection using bins.