Point-of-care colorimetric detection with a smartphone
Point-of-care colorimetric detection with a smartphone
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DOI：
10.1039/c2lc40741
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发表时间：
2012-01-01
期刊：
影响因子：
6.1
通讯作者：
Papautsky, Ian
中科院分区：
文献类型：
--
作者：
Shen, Li;Hagen, Joshua A.;Papautsky, Ian
基于纸张的免疫测定正在成为强大且低成本的诊断工具，特别是在资源有限的环境中。用于量化这些测定的廉价方法已经显示使用台式扫描仪，其缺乏便携性，和相机，其遭受不断变化的环境光条件。在这项工作中，我们介绍了一种新的方法，量化颜色的比色诊断分析与智能手机，允许在广泛的环境条件下的高精度测量，使其成为一个真正的便携式系统。我们使用色度值来构建分析物浓度的校准曲线，而不是直接使用智能手机相机拍摄的彩色图像的红色、绿色和蓝色（RGB）强度。我们证明了这种方法在pH测量中的高精度，线性响应范围为1-12。这些结果与使用台式扫描仪或硅光电探测器报告的结果相当。为了使该方法适用于不同的照明条件下，我们开发了一种校准技术，以补偿测量误差，由于环境光的变化。这种技术适用于许多常见的光源，例如太阳光、荧光灯或智能手机LED灯。最终，整个方法可以集成在一个“应用程序”中，以实现一键阅读，使我们的智能手机为基础的方法无需任何专业培训或复杂的仪器操作。
Paper-based immunoassays are becoming powerful and low-cost diagnostic tools, especially in resource-limited settings. Inexpensive methods for quantifying these assays have been shown using desktop scanners, which lack portability, and cameras, which suffer from the ever changing ambient light conditions. In this work, we introduce a novel approach of quantifying colors of colorimetric diagnostic assays with a smartphone that allows high accuracy measurements in a wide range of ambient conditions, making it a truly portable system. Instead of directly using the red, green, and blue (RGB) intensities of the color images taken by a smartphone camera, we use chromaticity values to construct calibration curves of analyte concentrations. We demonstrate the high accuracy of this approach in pH measurements with linear response ranges of 1-12. These results are comparable to those reported using a desktop scanner or silicon photodetectors. To make the approach adoptable under different lighting conditions, we developed a calibration technique to compensate for measurement errors due to variability in ambient light. This technique is applicable to a number of common light sources, such as sun light, fluorescent light, or smartphone LED light. Ultimately, the entire approach can be integrated in an "app" to enable one-click reading, making our smartphone based approach operable without any professional training or complex instrumentation.