A Novel Acinetobacter baumannii Bacteriophage Endolysin LysAB54 With High Antibacterial Activity Against Multiple Gram-Negative Microbes.
A Novel Acinetobacter baumannii Bacteriophage Endolysin LysAB54 With High Antibacterial Activity Against Multiple Gram-Negative Microbes.
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一种新型鲍曼不动杆菌噬菌体内溶素LysAB54，对多种革兰氏阴性微生物具有高抗菌活性
DOI：
10.3389/fcimb.2021.637313
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发表时间：
2021
影响因子：
5.7
通讯作者：
Yang H
中科院分区：
文献类型：
--
作者：
Khan FM;Gondil VS;Li C;Jiang M;Li J;Yu J;Wei H;Yang H
多重耐药鲍曼不动杆菌和其他致病性革兰氏阴性菌的快速传播和出现促使科学家和临床医生寻找传统抗生素的替代治疗药物。在本研究中，A.分离并鉴定鲍曼不动杆菌噬菌体p54。形态学和基因组分析表明，噬菌体p54属于肌尾病毒科，基因组大小为165，813 bp。一种新的内溶素，即LysAB 54，显示与其他众所周知的相关内溶素的低相似性，克隆，表达，并从噬菌体p54的特征。LysAB 54对多药耐药菌A.鲍曼不动杆菌和其它革兰氏阴性菌，包括铜绿假单胞菌、肺炎克雷伯氏菌和大肠杆菌，在不存在外膜透化剂的情况下。基于所有这些观察结果，LysAB 54可以代表用于治疗多重耐药革兰氏阴性超级细菌的潜在药剂。
The rapid spread and emergence of multidrug-resistant Acinetobacter baumannii and other pathogenic Gram-negative bacteria spurred scientists and clinicians to look for alternative therapeutic agents to conventional antibiotics. In the present study, an A. baumannii bacteriophage p54 was isolated and characterized. Morphological and genome analysis revealed that bacteriophage p54 belongs to Myoviridae family with a genome size of 165,813 bps. A novel endolysin, namely LysAB54, showing low similarity with other well-known related endolysins, was cloned, expressed, and characterized from the bacteriophage p54. LysAB54 showed significant bactericidal activity against multidrug-resistant A. baumannii and other Gram-negative bacteria, including Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli, in the absence of outer membrane permeabilizers. Based on all those observations, LysAB54 could represent a potential agent for the treatment of multidrug-resistant Gram-negative superbugs.