Assessment of nematodes in Punjab Urial (Ovis vignei punjabiensis) population in Kalabagh Game Reserve: development of a DNA barcode approach
Assessment of nematodes in Punjab Urial (Ovis vignei punjabiensis) population in Kalabagh Game Reserve: development of a DNA barcode approach
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DOI：
10.1007/s10344-019-1298-y
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发表时间：
2019-08-01
影响因子：
2
通讯作者：
Shehzad, Wasim
中科院分区：
文献类型：
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作者：
Bajwa, Amna Arshad;Cuff, Jordan Patrick;Shehzad, Wasim
旁遮普乌拉尔羊是巴基斯坦的一种野生绵羊，被世界自然保护联盟视为易受伤害的物种。对尿液种群的主要威胁包括栖息地丧失和偷猎，导致其种群数量严重下降。线虫感染也可能危及尿路生存，但人们对旁遮普尿路胃肠道线虫知之甚少。在这项研究中，开发了一种新的DNA条形码方法，以ITS-I为靶区，设计了一对引物来扩增小型反刍动物经常报告的线虫物种。这对新的引物在计算机和体外进行了验证，随后用于确定巴基斯坦绵瓦里地区Kala Bagh猎物保护区旁遮普省尿样中线虫的存在。DNA条码显示，在旁遮普邦尿液中，缠绕血吸虫(73.91%)、绵羊毛滴虫(16.30%)和阿克苏毛圆线虫(3.26%)的患病率较高。这项研究表明，新的DNA条形码方法是从旁遮普尿液粪便样本中检测线虫寄生虫的可靠工具。该方法可用于检测野生和家养寄主的线虫感染情况，用于监测和种群保护。
Punjab urial (Ovis vignei punjabiensis) is a wild sheep of Pakistan, considered a vulnerable species by IUCN. Major threats to urial populations include habitat loss and poaching, causing severe declines in its population. Nematode infections may also compromise urial survival, but little is known about Punjab urial gastrointestinal nematodes. In this study, a novel DNA barcoding approach was developed using ITS-I as a target region, with a primer pair designed to amplify frequently reported nematode species for small ruminants. The novel primer pair was validated in silico and in vitro and subsequently used to determine the presence of nematodes in Punjab urial samples from Kala Bagh Game Reserve, District Mianwali (Pakistan). DNA barcoding revealed a higher prevalence of Haemonchus contortus (73.91%), Trichuris ovis (16.30%) and Trichostrongylus axei (3.26%) in Punjab urial. This study demonstrates that the novel DNA barcoding approach is a robust tool to detect nematode parasites from faecal samples of Punjab urial. This method can be used to detect nematode infections in wild and domestic hosts for surveillance and population conservation.