Immuno-localization of ESCRT Proteins in Virus-Infected Cells by Fluorescence and Electron Microscopy
Immuno-localization of ESCRT Proteins in Virus-Infected Cells by Fluorescence and Electron Microscopy
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通过荧光和电子显微镜对病毒感染细胞中的 ESCRT 蛋白进行免疫定位
DOI：
10.1007/978-1-4939-9492-2_6
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Morita E.
中科院分区：
文献类型：
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作者：
Tabata K.;Nara A.;Omori H. ;Morita E.
许多包膜病毒利用细胞ESCRT途径进行出芽，甚至黄病毒，其在来自内质网（ER）的复制细胞器内形成病毒颗粒。在本节中，我们将介绍通过免疫荧光显微镜和免疫电子显微镜（免疫EM）检测病毒感染细胞中几种ESCRT亚基蛋白的方法。我们还介绍了一种新的方法;相关光学显微镜和电子显微镜（CLEM），它允许观察目标结构与高分辨率EM和荧光标记。
Many enveloped viruses utilize the cellular ESCRT pathway for budding, even flaviviruses, which form viral particles inside replication organelles derived from the endoplasmic reticulum (ER). In this section, we introduce methods for detecting several ESCRT subunit proteins in virus-infected cells by immunofluorescence microscopy and immunoelectron microscopy (immuno-EM). We also introduce a new method; correlative light microscopy and electron microscopy (CLEM), which allows the observation of target structures with both high-resolution EM and fluorescence labeling.