Granulosal and thecal expression of bone morphogenetic protein- and activin-binding protein mRNA transcripts during bovine follicle development and factors modulating their expression in vitro
Granulosal and thecal expression of bone morphogenetic protein- and activin-binding protein mRNA transcripts during bovine follicle development and factors modulating their expression in vitro
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DOI：
10.1530/rep-11-0150
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发表时间：
2011-10-01
期刊：
影响因子：
3.8
通讯作者：
Knight, Phil G.
中科院分区：
文献类型：
--
作者：
Glister, Claire;Satchell, Leanne;Knight, Phil G.
有证据支持转化生长因子β超家族成员，包括激活素和骨形态发生蛋白（BMP）在卵泡发育中的局部作用。这些配体到信号受体的通路可能是由细胞外结合蛋白（BP）调节的。在这项研究中，我们比较了四种bp （chordin, gremlin， noggin和follistatin）在发育中的牛窦卵泡（1-18 mm）颗粒（GC）和内腔（TC）室中的体外表达。同时观察FSH和IGF对体外培养GC细胞BMP和BP表达的影响，以及LH和BMP对体外培养TC细胞BP表达的影响。四种BP转录物的滤泡表达在GC区室高于TC区室（P < 0.001），免疫组织化学证实了这一发现。卵泡类型影响格莱姆林和卵泡抑素mRNA丰度（P < 0.01），对chordin、卵泡抑素和noggin有显著的细胞-卵泡类型交互作用。大“e活性”卵泡GC中Noggin转录物丰度低于“e非活性”卵泡GC (P < 0.05)，而follistatin mRNA水平高于“e非活性”卵泡GC （P < 0.01）。FSH通过GC增强CYP19、FSHR、INHBA和卵泡抑制素，但不影响BMP或BMP- bp的表达。IGF增加CYP19和卵泡抑素，降低BMP4、noggin和gremlin，但不影响chordin或FSHR mRNA水平。LH增加TC雄激素分泌，但对BMP和BP表达无影响。bmp均抑制TC雄激素的产生，同时使chordin、noggin和gremlin mRNA水平升高20倍（P < 0.01）。这些发现支持了细胞外BP（主要来自GC）对卵泡内BMP/激活素信号的调节的假设。BMP对鞘膜BP表达的增强暗示了一种防止过度信号传导的自我调节反馈作用。复制（2011）142 581-591
Evidence supports local roles for transforming growth factor beta superfamily members including activins and bone morphogenetic proteins (BMP) in follicle development. Access of these ligands to signalling receptors is likely modulated by extracellular binding proteins (BP). In this study, we compared ex vivo expression of four BPs (chordin, gremlin, noggin and follistatin) in granulosal (GC) and theca interna (TC) compartments of developing bovine antral follicles (1-18 mm). Effects of FSH and IGF on BMP and BP expression by cultured GC, and effects of LH and BMPs on BP expression by cultured TC were also examined. Follicular expression of all four BP transcripts was higher in GC than TC compartments (P < 0.001) a finding confirmed by immunohistochemistry. Follicle category affected (P < 0.01) gremlin and follistatin mRNA abundance, with a significant cell-type x follicle category interaction for chordin, follistatin and noggin. Noggin transcript abundance was lower (P < 0.05) in GC of large 'E-active' than 'E-inactive' follicles while follistatin mRNA level was higher (P < 0.01). FSH enhanced CYP19, FSHR, INHBA and follistatin by GC without affecting BMP or BMP-BP expression. IGF increased CYP19 and follistatin, reduced BMP4, noggin and gremlin but did not affect chordin or FSHR mRNA levels. LH increased TC androgen secretion but had no effect on BMP or BP expression. BMPs uniformly suppressed TC androgen production whilst increasing chordin, noggin and gremlin mRNA levels up to 20-fold (P < 0.01). These findings support the hypothesis that extracellular BP, mostly from GC, contribute to the regulation of intrafollicular BMP/activin signalling. Enhancement of thecal BP expression by BMP implies an autoregulatory feedback role to prevent excessive signalling. Reproduction (2011) 142 581-591