Assessing the potential use of environmental DNA for multifaceted genetic monitoring of cetaceans: Example of a wandering whale in a highly disturbed bay area
Assessing the potential use of environmental DNA for multifaceted genetic monitoring of cetaceans: Example of a wandering whale in a highly disturbed bay area
复制标题
评估环境 DNA 在鲸目动物多方面基因监测中的潜在用途：以高度干扰的海湾地区的流浪鲸为例
DOI：
10.1016/j.ecolind.2023.110125
复制
发表时间：
2023
影响因子：
6.9
通讯作者：
Min Li
中科院分区：
文献类型：
--
作者：
Shuai Zhang;Yiting Cao;Bin Chen;Peiwen Jiang;Liang Fang;Hongting Li;Zuozhi Chen;Shannan Xu;Min Li
水体环境DNA（eDNA）采样是一种对水生动物物种进行全面、无创监测的有效方法。然而，很少有报告将其应用于鲸目动物物种。2021年6月29日，一头鲸鱼（绰号小布）出现在中国广东省大鹏湾外海。我们使用eDNA技术获得了与这头鲸鱼有关的信息（例如，物种鉴定和食物资源），并追踪其可能的来源。从大鹏湾采集的eDNA扩增产物中获得了4条鲸鱼线粒体序列（12 S rDNA、16 S rDNA、细胞色素氧化酶亚基1和控制区）的片段;序列条形码显示这是一头伊甸鲸（Balaenoptera edeni edeniAnderson 1879）。对潜在猎物种类的分析表明，该鲸可能是在追踪猎物时进入大鹏湾的，主要是沙丁鱼（Sardinella lemuru，Sardinella gibbosaandSardinella jussieui）和鱼（Thryssa dussumieri，Thryssa vitrirostrisandThryssa kammalensis）。从大鹏湾邻近沃茨（即伶仃湾和大亚湾）采集的样本中检索eDNA元条形码数据，发现伊甸鲸在大鹏湾出现（2021年4月初）前最多2个月就已在伶仃湾外出现。总的来说，这项研究表明，eDNA是一种非常有效的非侵入性调查方法，可用于准确识别目标鲸目动物物种和猎物组成;它可用于监测受到严格法律的保护的巨型动物或监测条件未知的巨型动物。
Environmental DNA (eDNA) sampling of water is a powerful method for comprehensive and noninvasive monitoring of aquatic animal species. However, there have been few reports of its application to cetacean species. On June 29, 2021, a whale (nicknamed Xiaobu) appeared in Dapeng Bay off Guangdong Province, China. We used eDNA technology to obtain information related to this whale (e.g., species identification and food resources) and to trace its possible origin. Fragments of four whale mitochondrial sequences (12S rDNA, 16S rDNA, cytochromecoxidase subunit 1, and control region) were obtained from amplicons of eDNA collected in Dapeng Bay; sequence barcoding showed that this was an Eden’s whale (Balaenoptera edeni edeniAnderson 1879). Analysis of potential prey species (PPS) suggested that this whale might enter Dapeng Bay while tracking prey, mainly sardines (Sardinella lemuru,Sardinella gibbosaandSardinella jussieui) and anchovies (Thryssa dussumieri,Thryssa vitrirostrisandThryssa kammalensis). Retrieval of eDNA metabarcoding data from samples collected in waters adjacent to Dapeng Bay (i.e. Lingding Bay and Daya Bay) revealed that Eden’s whale had appeared outside Lingding Bay up to 2 months prior to the appearance of this whale in Dapeng Bay (early April 2021). Overall, this study showed that eDNA is a highly effective noninvasive survey method for the accurate identification of target cetacean species and prey composition; it can be used to monitor megafauna that are under strict legal protection or to monitor megafauna with unknown conditions.