Dual recognition of a C-G pyrimidine-purine inversion site:: synthesis and binding properties of triplex forming oligonucleotides containing 2′-aminoethoxy-5-methyl-1H-pyrimidin-2-one ribonucleosides
Dual recognition of a C-G pyrimidine-purine inversion site:: synthesis and binding properties of triplex forming oligonucleotides containing 2′-aminoethoxy-5-methyl-1H-pyrimidin-2-one ribonucleosides
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DOI：
10.1016/s0040-4039(03)01135-3
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发表时间：
2003-06-30
影响因子：
1.8
通讯作者：
Leumann, CJ
中科院分区：
文献类型：
--
作者：
Buchini, S;Leumann, CJ
合成了一种新的核糖核苷类似物，它结合了两种修饰，即核糖上的2 '-氨基乙氧基侧链和5-甲基-1H-嘧啶-2-酮（T-4 H）单元作为碱基置换。将该结构单元掺入形成三链体的寡核苷酸中，并研究了其与DNA双链体靶中CG倒位位点的结合特性。数据。清楚地表明T-4 H碱基选择性识别CG碱基对，而氨基乙氧基链增加了三螺旋的整体稳定性。(C)2003爱思唯尔科技有限公司版权所有。
The synthesis of a new ribonucleoside analogue, which combines two modifications, namely a 2'-aminoethoxy side-chain on the ribose and a 5-methyl-1H-pyrimidin-2-one (T-4H) unit as a base replacement, is presented. This building block was incorporated into triplex forming oligonucleotides and the binding properties to CG inversion sites in DNA duplex targets were studied. The data. clearly show that the T-4H base selectively recognizes the CG base-pair, while the aminoethoxy chain adds to the overall stability of the triple helix. (C) 2003 Elsevier Science Ltd. All rights reserved.