Changes in circulating and testicular levels of inhibin A and B and activin A during postnatal development in the rat
Changes in circulating and testicular levels of inhibin A and B and activin A during postnatal development in the rat
复制标题
DOI：
10.1210/en.2003-1036
复制
发表时间：
2004-07-01
期刊：
影响因子：
4.8
通讯作者：
de Kretser, DM
中科院分区：
文献类型：
--
作者：
Buzzard, JJ;Loveland, KL;de Kretser, DM
本研究用Northern分析和原位杂交方法研究了大鼠睾丸抑制素/激活素亚基的表达水平，用酶联免疫吸附分析法(ELISA)测定了大鼠出生后发育过程中血清和睾丸抑制素A、B和激活素A的水平。我们发现，在出生后的整个生命过程中，血清抑制素A水平低于4pg/ml。成年男性血清抑制素B水平在产后40天达到高峰，为572±/-119pg/ml(平均值+/-SE)，之后下降至182+/-35pg/ml。血清激活素A从出生后第6天的294+/-29pg/ml下降到成熟期的132+/-27pg/ml。在睾丸内，抑制素A水平从第15天的0.330+/-0.108 ng/g下降到成熟时的不到0.004 ng/g。抑制素B水平在第6天达到峰值，为43.9+/-4.2 ng/g，成熟时降至1.6+/-0.13 ng/g。睾丸激活素A水平从第6天的18.6+/-2.2 ng/g下降到成熟时的0.094+/-0.013 ng/g。睾丸抑制素/激活素亚基的Northern图谱与ELISA法显示的免疫反应水平相关。原位杂交表明，β(A)和β(B)亚基的表达主要局限于曲细精管，尤其是支持细胞、精原细胞和初级精母细胞。这些数据支持这样的观点，即抑制素B是雄性大鼠体内的主要抑制素，其水平与支持细胞的数量和活性有关。此外，在支持细胞增殖和精子发生的开始阶段，激活素A的局部浓度很高，这支持了它的作用，因为它是睾丸发育和功能的调节器。
This study describes the testicular levels of inhibin/activin subunits by Northern analysis and in situ hybridization and serum and testicular levels of inhibins A and B and activin A by enzyme linked immunosorbent assays ( ELISA) during postnatal development in the rat. We show that serum inhibin A levels are less than 4 pg/ml throughout postnatal life. Serum inhibin B levels peak at 572 +/- 119 pg/ml ( mean +/- SE) at d 40 post partum ( pp) before falling to 182 +/- 35 pg/ml in mature males. Serum activin A decreases from 294 +/- 29 pg/ml at d 6 to 132 +/- 27 pg/ml at maturity. Within the testis, inhibin A levels fall from 0.330 +/- 0.108 ng/g at d 15 to less than 0.004 ng/g at maturity. Inhibin B levels peak at 43.9 +/- 4.2 ng/g at d 6 before falling to 1.6 +/- 0.13 ng/g at maturity. Testicular activin A levels fall from 18.6 +/- 2.2 ng/g at d 6 to 0.094 +/- 0.013 ng/g at maturity. Northern profiles of testicular inhibin/activin subunits correlate with immunoreactive levels demonstrated by ELISA. In situ hybridization suggests that beta(A) and beta(B) subunit expression is largely restricted to the seminiferous tubule, particularly Sertoli cells, spermatogonia, and primary spermatocytes. These data support the view that inhibin B is the major inhibin in the male rat and that levels relate to Sertoli cell number and activity. Furthermore, the demonstration of high local concentrations of activin A during the period of Sertoli cell proliferation and the onset of spermatogenesis support its proposed role because a modulator of testicular development and function.