Variations in kinetics of alkaline phosphatase in sediments of eutrophic, shallow, Chinese lakes
Variations in kinetics of alkaline phosphatase in sediments of eutrophic, shallow, Chinese lakes
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DOI：
10.1007/s10750-006-0503-7
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发表时间：
2007-05
期刊：
影响因子：
2.6
通讯作者：
Yiyong Zhou;Xiuyun Cao;Chunlei Song;Jianqiu Li;Guoyuan Chen;Liang Peng
中科院分区：
文献类型：
--
作者：
Yiyong Zhou;Xiuyun Cao;Chunlei Song;Jianqiu Li;Guoyuan Chen;Liang Peng
研究了中国浅水湖（东湖）沉积物中碱性磷酸酶的动力学。从空间上看，20个采样点中，表层沉积物中碱性磷酸酶的Vmax和Km值在邻近叶绿素浓度最高的地点的区域较高。垂直方向上，除了表面的预期最大值外，在中间沉积深度处还存在 Vmax 峰值。一些抑制剂，如CuSO4、ZnSO4和Na2WO4，对间质水和沉积物中碱性磷酸酶的动力学表现出显着不同的影响。此外，太湖间质水和沉积物中的碱性磷酸酶对Na2WO4的响应方式不同。这些观察结果表明，该酶被固定在沉积物中，随着富营养化的加速，沉积物变得更加稳定，正如东湖最高水柱叶绿素浓度所对应的沉积物中最高的碱性磷酸酶活性（APA）所表明的那样。
Kinetics of alkaline phosphatase in sediments of a shallow Chinese freshwater lake (Lake Donghu) were investigated. Spatially, among 20 sites sampled,VmaxandKmvalues of alkaline phosphatase in surface sediments were higher in the zone adjacent to sites with the highest chlorophyllaconcentrations. Vertically, there was a peak inVmaxat intermediate sediment depths in addition to the expected maximum at the surface. Some inhibitors, such as CuSO4, ZnSO4and Na2WO4, showed significantly different effects on kinetics of alkaline phosphatase in interstitial water and sediments. Moreover, alkaline phosphatase in interstitial water and sediments responded to Na2WO4in different ways in Lake Taihu. These observations imply that the enzyme is immobilized in sediments, which became more stable with accelerated eutrophication, as suggested by highest alkaline phosphatase activity (APA) in sediments corresponding with highest water column chlorophyllaconcentrations in Lake Donghu.