Characterisation of pregnancy losses after embryo transfer by measuring plasma progesterone and bovine pregnancy-associated glycoprotein-1 concentrations
Characterisation of pregnancy losses after embryo transfer by measuring plasma progesterone and bovine pregnancy-associated glycoprotein-1 concentrations
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DOI：
10.1016/j.tvjl.2012.02.020
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发表时间：
2012-10-01
影响因子：
2.2
通讯作者：
Szenci, O.
中科院分区：
文献类型：
--
作者：
Breukelman, S. P.;Perenyi, Z.;Szenci, O.
本分析的目的是确定牛胚胎移植（ET）后的妊娠丢失（PL）是否与ET期间和ET后不久的母体孕酮（P4）浓度以及妊娠第25-35天血浆中的母体牛妊娠相关糖蛋白-1（bPAG-1）浓度相关。超数排卵后体内（n = 115）、体外（n = 44）或合成培养基中培养的卵母细胞（n = 101）产生胚胎（n = 260）。总的来说，PL为56.9%（148），三个胚胎产生组之间的产犊率没有显著差异。三组妊娠第7-14天的P4浓度无差异，正在进行的妊娠和中断的妊娠之间也无差异。在妊娠第25天和第35天之间，bPAG-1浓度不受胚胎生产的影响，但在第26天和第120天之间患有PL的牛中，可以检测到四种bPAG-1谱。在第25天和第32天之间，bPAG-1浓度受到PL的影响，在第26天和第120天之间发生PL的动物中，bPAG-1浓度显著低于后期流产或足月产犊的动物。早期P4浓度表明，母体黄体因素不负责PL，这似乎是由受损的孕体发育（无论胚胎类型），反映了低母体bPAG-1浓度胚胎死亡前。(C)2012爱思唯尔有限公司保留所有权利。
The aim of this analysis was to determine whether pregnancy loss (PL) after embryo transfer (ET) in cattle was related to maternal progesterone (P4) concentrations during and shortly after ET, and maternal bovine pregnancy-associated glycoprotein-1 (bPAG-1) concentrations in plasma at days 25-35 of gestation. Embryos (n = 260) were produced either in vivo after superovulation (n = 115), or in vitro from oocytes (obtained with ovum pick-up) in co-culture (n = 44) or cultured in a synthetic medium (n = 101).Overall, PL was 56.9% (148) and no significant differences occurred in calving rate among the three embryo production groups. There was no difference in P4 concentrations on days 7-14 of gestation in the three groups, nor between ongoing and interrupted pregnancies. Between days 25 and 35 of pregnancy, bPAG-1 concentrations were unaffected by embryo production, but in cattle that had PL between days 26 and 120, four bPAG-1 profiles could be detected. Between days 25 and 32, bPAG-1 concentrations were influenced by PL, and concentrations were significantly lower in animals in which PL occurred between days 26 and 120 than in those animals that aborted later or calved at term. Early P4 concentrations suggested that maternal luteal factors were not responsible for PL which appeared to be caused by impaired conceptus development (regardless of embryo type) as reflected by low maternal bPAG-1 concentrations prior to embryonic death. (C) 2012 Elsevier Ltd. All rights reserved.