SPORULATION AND TOXIN PRODUCTION BY BACILLUS-THURINGIENSIS VAR ISRAELENSIS IN CADAVERS OF MOSQUITO LARVAE (DIPTERA, CULICIDAE)
SPORULATION AND TOXIN PRODUCTION BY BACILLUS-THURINGIENSIS VAR ISRAELENSIS IN CADAVERS OF MOSQUITO LARVAE (DIPTERA, CULICIDAE)
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DOI：
10.1016/0022-2011(85)90066-7
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发表时间：
1985-01-01
影响因子：
3.4
通讯作者：
FEDERICI, BA
中科院分区：
文献类型：
--
作者：
ALY, C;MULLA, MS;FEDERICI, BA
室内实验结果表明，苏云金芽孢杆菌（Bacillus thuringiensis var.）Israelensis，可在蚊幼虫尸体上生长，形成孢子，并产生毒素。以.埃及人，孢子计数从2x上升102/尸体，处理后4小时至1.4 ×105/尸，而A.每具尸体的白对虾孢子计数从2.2 × 104增加到2.5 × 104。103至3.2 ×治疗后72小时为105。幼虫尸体的生物测定表明，B的毒性与产孢有关。苏云Israelensis在处理后约72小时达到最大水平。这些结果表明，在适当的条件下，B.图林根变种Israelensis可以使用幼虫尸体中可获得的基质用于生长和孢子形成。
Laboratory experiments with 4th-instar larvae of Aedes aegypti and Anopheles albimanus (Diptera: Culicidae) demonstrated that the entomocidal bacterium, Bacillus thuringiensis var. israelensis, can grow vegetatively, sporulate, and produce toxin in cadavers of mosquito larvae. In A. aegypti, spore counts rose from 2 .times. 102/cadaver 4 h after treatment to 1.4 .times. 105/cadaver approximately 72 h later, whereas in A. albimanus spore counts per cadaver increased from 2.2 .times. 103 between 4 and 24 h to 3.2 .times. 105 at 72 h post-treatment. Bioassays of larval cadavers indicated that toxicity associated with sporulation of B. thuringiensis var. israelensis reached a maximum level approximately 72 h after treatment. These results demonstrate that under appropriate conditions B. thuringinesis var. israelensis can use the substrates available in larval cadavers for growth and sporulation.