Hyperoxic exposure leads to cell death in the developing brain
Hyperoxic exposure leads to cell death in the developing brain
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DOI：
10.1016/j.braindev.2008.01.010
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发表时间：
2008-10-01
影响因子：
1.7
通讯作者：
Yilmaz, Osman
中科院分区：
文献类型：
--
作者：
Yis, Uluc;Kurul, Semra Hiz;Yilmaz, Osman
早产儿在以后的生活中有很高的运动和认知障碍发生率。超生理氧浓度通常用于新生儿重症监护病房，并引起早产儿肺和视网膜损伤。由于高氧对发育中的大脑的影响很少，我们研究了高氧对该组织的影响。Wistar大鼠幼崽从出生到第5天暴露在21%或80%的氧气中。采用免疫组织化学和ELISA细胞死亡法检测海马CA1和齿状回、前额叶皮质、顶叶皮质、骨下和脾后皮质的神经元密度和凋亡情况。高氧组脑区神经元密度明显降低。此外。通过ELISA细胞死亡和TUNEL检测，我们观察到发育中的脑细胞死亡增加。我们的研究结果表明，高氧可诱导发育中的大鼠脑细胞死亡。这可能是导致早产儿后期运动和认知障碍的重要机制之一。(c) 2008 Elsevier B.V.版权所有
Premature infants have high incidence of motor and cognitive impairment in later life. Supraphysiological oxygen concentrations are routinely used in neonatal intensive care units and elicit injury to premature lungs and retina. Since the effects of hyperoxia on the developing brain are scarce, we Studied the effects of hi h oxygen on this tissue. Wistar rat pups were exposed from birth until day 5 to 21% or 80% oxygen. The neuronal density and apoptosis in CA1 and dentate gyrus of hippocampus, prefrontal cortex, parietal cortex, subiculum, and retrosplenial cortex were assessed by immunohistochemistry and ELISA cell death assay. Neuronal density of the investigated brain areas were significantly decreased in the hyperoxia group. Furthermore. using ELISA cell death and TUNEL assays, we observed increased cell death in the developing brain. Our results show that hyperoxia induces cell death in the developing rat brain. This may be one of the important mechanisms that cause motor and cognitive impairment in later life of premature infants. (c) 2008 Elsevier B.V. All rights reserved.