Heparan Sulfate Regrowth Profiles Under Laminar Shear Flow Following Enzymatic Degradation.
Heparan Sulfate Regrowth Profiles Under Laminar Shear Flow Following Enzymatic Degradation.
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DOI：
10.1007/s12195-013-0273-z
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发表时间：
2013-06
影响因子：
2.8
通讯作者：
Dewey, C. Forbes, Jr.
中科院分区：
文献类型：
--
作者：
Giantsos-Adams, Kristina M.;Koo, Andrew Jia-An;Song, Sukhyun;Sakai, Jiro;Sankaran, Jagadish;Shin, Jennifer H.;Garcia-Cardena, Guillermo;Dewey, C. Forbes, Jr.
动脉局部血流动力学剪切应力波形决定了内皮细胞的表型。在循环动脉粥样硬化区内皮上观察到的顶端糖萼层的减少表明糖萼可能在决定动脉粥样硬化斑块形成中起核心作用。然而，细胞使其糖萼适应环境的能力的动力学尚未得到定量解决。在这里，我们报告了高剪切应力开始后，HUVECs糖萼中的硫酸肝素成分与静态培养细胞相比增加了1.4倍，时间常数为19小时。细胞形态学实验表明，细胞需要12小时才能伸长，但只有在36小时后细胞才能达到与流载体的最大排列。我们的研究结果表明，酶降解后，硫酸肝素在流动条件下在12小时内恢复到细胞表面，而在静态条件下需要20小时。我们还提出了一个模型来描述胞吞作用和胞吐作用对根尖硫酸肝素表达的贡献。HS再生动力学从静态到高剪切EC表型的变化意味着内吞噬和外胞膜周转速率的差异。
The local hemodynamic shear stress waveforms present in an artery dictate the endothelial cell phenotype. The observed decrease of the apical glycocalyx layer on the endothelium in atheroprone regions of the circulation suggests that the glycocalyx may have a central role in determining atherosclerotic plaque formation. However, the kinetics for the cells’ ability to adapt its glycocalyx to the environment have not been quantitatively resolved. Here we report that the heparan sulfate component of the glycocalyx of HUVECs increases by 1.4-fold following the onset of high shear stress, compared to static cultured cells, with a time constant of 19 h. Cell morphology experiments show that 12 h are required for the cells to elongate, but only after 36 h have the cells reached maximal alignment to the flow vector. Our findings demonstrate that following enzymatic degradation, heparan sulfate is restored to the cell surface within 12 h under flow whereas the time required is 20 h under static conditions. We also propose a model describing the contribution of endocytosis and exocytosis to apical heparan sulfate expression. The change in HS regrowth kinetics from static to high-shear EC phenotype implies a differential in the rate of endocytic and exocytic membrane turnover.
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DOI：
10.1152/ajpheart.00592.2005
发表时间：
2006-01-01
影响因子：
4.8
作者：
Gouverneur, M;Spaan, JAE;Vink, H
通讯作者：
Vink, H
DOI：
10.1016/j.bbrc.2007.01.137
发表时间：
2007-03-30
影响因子：
3.1
作者：
Pahakis, Manolis Y.;Kosky, Jason R.;Tarbell, John M.
通讯作者：
Tarbell, John M.
影响因子：
3.1
作者：
Gao L;Lipowsky HH
通讯作者：
Lipowsky HH
影响因子：
4.1
作者：
Fuki, IV;Meyer, ME;Williams, KJ
通讯作者：
Williams, KJ
影响因子：
3.7
作者：
Khan, Abdul Ghafoor;Pickl-Herk, Angela;Blaas, Dieter
通讯作者：
Blaas, Dieter