Proteomic Analysis of the Excretory and Secretory Proteins of Haemonchus contortus (HcESP) Binding to Goat PBMCs In Vivo Revealed Stage-Specific Binding Profiles.
Proteomic Analysis of the Excretory and Secretory Proteins of Haemonchus contortus (HcESP) Binding to Goat PBMCs In Vivo Revealed Stage-Specific Binding Profiles.
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DOI：
10.1371/journal.pone.0159796
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Gadahi JA;Wang S;Bo G;Ehsan M;Yan R;Song X;Xu L;Li X
捻转血矛线虫是一种寄生性胃肠道线虫，其排泄物和分泌产物（HcESP）与宿主细胞广泛相互作用。在这项研究中，我们使用液相色谱-串联质谱法报告了不同发育阶段的 HcESP 蛋白质与体内山羊外周血单核细胞 (PBMC) 的相互作用。使用 SEQUEST 搜索从 H. contortus 蛋白质数据库中鉴定出总共 407 个在不同时间点与山羊 PBMC 相互作用的 HcESP。与成虫早期和晚期相比，捻转 H. contortus 的 L4 和 L5 阶段代表了更高比例的已鉴定蛋白质。鉴定了阶段特异性相互作用蛋白和多个阶段共有的蛋白质。四十七个相互作用的蛋白质在所有阶段之间共享。已鉴定的山羊 PBMC 相互作用蛋白的基因本体 (GO) 分布在所有发育阶段几乎相同，具有高度的结合和催化活性代表性。细胞、代谢和单一生物体过程也被注释为主要生物过程，但有趣的是，与 L4 和成体阶段相比，更多的蛋白质在 L5 阶段被注释为定位过程。基于同源蛋白的聚类，我们改进了在不同发育阶段鉴定的未注释蛋白的功能注释。通过 STRING 蛋白聚类分析鉴定了一些未命名的 H. contortus ATP 结合盒蛋白，包括 ADP-核糖基化因子和 P-糖蛋白-9。
Haemonchus contortus is a parasitic gastrointestinal nematode, and its excretory and secretory products (HcESPs) interact extensively with the host cells. In this study, we report the interaction of proteins from HcESPs at different developmental stages to goat peripheral blood mononuclear cells (PBMCs) in vivo using liquid chromatography-tandem mass spectrometry. A total of 407 HcESPs that interacted with goat PBMCs at different time points were identified from a H. contortus protein database using SEQUEST searches. The L4 and L5 stages of H. contortus represented a higher proportion of the identified proteins compared with the early and late adult stages. Both stage-specific interacting proteins and proteins that were common to multiple stages were identified. Forty-seven interacting proteins were shared among all stages. The gene ontology (GO) distributions of the identified goat PBMC-interacting proteins were nearly identical among all developmental stages, with high representation of binding and catalytic activity. Cellular, metabolic and single-organism processes were also annotated as major biological processes, but interestingly, more proteins were annotated as localization processes at the L5 stage than at the L4 and adult stages. Based on the clustering of homologous proteins, we improved the functional annotations of un-annotated proteins identified at different developmental stages. Some unnamed H. contortus ATP-binding cassette proteins, including ADP-ribosylation factor and P-glycoprotein-9, were identified by STRING protein clustering analysis.