Synthesis of 70K stress protein by human leukocytes: effect of exercise in the heat.
Synthesis of 70K stress protein by human leukocytes: effect of exercise in the heat.
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人类白细胞合成 70K 应激蛋白：高温运动的影响。
DOI：
10.1152/jappl.1991.70.1.466
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Moseley,PL
中科院分区：
文献类型：
--
作者：
Ryan,AJ;Gisolfi,CV;Moseley,PL
为了确定70，000-Da（70 K）应激蛋白合成的再诱导是否可以用作热历史和/或热热人类中70 K应激蛋白的细胞水平的测定，在运动2小时之前和之后获得白细胞，然后在37或41 ℃下孵育。5名健康男性完成了2小时的跑步机运动，包括以4-6 km/h的速度跑步30-45 min，然后步行75-90 min，爬2-10%的坡度。该运动回合由两名受试者在热（46摄氏度，15%相对湿度）环境条件下进行，并且由五名受试者在较冷（30摄氏度，40%相对湿度）环境条件下进行。运动导致直肠温度（Tre）低于40摄氏度并没有改变白细胞在41摄氏度下孵育合成的70 K应激蛋白的量。相比之下，运动导致Tre高于40摄氏度，减少了在41摄氏度孵育的白细胞合成的70 K应激蛋白的量。蛋白质免疫印迹，探测与诱导72 K应激蛋白的特异性抗体，表明35 S标记的70 K应激蛋白在这些postexercise白细胞样本中的减少发生没有显着升高这种蛋白质。在40摄氏度下体外孵育人白细胞15-120分钟，以时间依赖性方式减少了随后41摄氏度热应激期间合成的70 K应激蛋白的量。当放线菌酮存在于40 ℃预孵育过程中时，41 ℃处理的白细胞中70 K应激蛋白合成的减少被废除。（250字处删节）
To determine whether reinduction of 70,000-Da (70K) stress protein synthesis could be used as an assay for thermal history and/or cellular levels of 70K stress protein in hyperthermic humans, leukocytes were obtained before and after 2 h of exercise and then incubated at 37 or 41 degrees C. Five healthy males completed 2 h of treadmill exercise consisting of running at 4–6 km/h for 30–45 min followed by 75–90 min of walking up a 2–10% grade. This exercise bout was performed by two subjects in hot (46 degrees C, 15% relative humidity) and by five subjects in cooler (30 degrees C, 40% relative humidity) environmental conditions. Exercise resulting in rectal temperature (Tre) less than 40 degrees C did not alter the amount of 70K stress protein synthesized by leukocytes incubated at 41 degrees C. In contrast, exercise resulting in Tre greater than 40 degrees C reduced the amount of 70K stress protein synthesized by leukocytes incubated at 41 degrees C. A protein immunoblot, probed with an antibody specific for the inducible 72K stress protein, showed that the reduction of 35S-labeled 70K stress protein in these postexercise leukocyte samples occurred without marked elevations of this protein. In vitro incubation of human leukocytes at 40 degrees C for 15–120 min reduced, in a time-dependent manner, the amount of 70K stress protein synthesized during a subsequent 41 degrees C heat stress. This reduction of 70K stress protein synthesis in 41 degrees C-treated leukocytes was abolished when cycloheximide was present during the 40 degrees C preincubation.(ABSTRACT TRUNCATED AT 250 WORDS)