Direct targets of the D. melanogaster DSXF protein and the evolution of sexual development
Direct targets of the D. melanogaster DSXF protein and the evolution of sexual development
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DOI：
10.1242/dev.065227
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发表时间：
2011-07-01
期刊：
影响因子：
4.6
通讯作者：
Baker, Bruce S.
中科院分区：
文献类型：
--
作者：
Luo, Shengzhan D.;Shi, Guang W.;Baker, Bruce S.
揭示双性 (dsx) 和无果 (fru) 的直接调控目标对于了解它们如何调节黑腹果蝇的性发育、形态发生、分化和成体功能（包括行为）至关重要。使用改进的 DamID 方法，我们在基因组中鉴定了 650 个 DSX 结合区域，然后从中提取了最佳回文 13 bp DSX 结合序列。该序列在体内具有功能，并且每个位置的碱基同一性对于体外 DSX 结合很重要。此外，该序列在黑腹果蝇的基因组中（58 个拷贝，而随机预期的大约 3 个拷贝）和其他 11 个已测序果蝇物种以及其他一些双翅目动物的基因组中也有所丰富。二十三个基因与 DSX 结合的体内峰和最佳 DSX 结合序列相关，因此几乎肯定是直接的 DSX 靶标。这 23 个基因与最佳 DSX 结合位点的关联用于检查 DSX 及其在昆虫中的靶标发生的进化变化。
Uncovering the direct regulatory targets of doublesex (dsx) and fruitless (fru) is crucial for an understanding of how they regulate sexual development, morphogenesis, differentiation and adult functions (including behavior) in Drosophila melanogaster. Using a modified DamID approach, we identified 650 DSX-binding regions in the genome from which we then extracted an optimal palindromic 13 bp DSX-binding sequence. This sequence is functional in vivo, and the base identity at each position is important for DSX binding in vitro. In addition, this sequence is enriched in the genomes of D. melanogaster (58 copies versus approximately the three expected from random) and in the 11 other sequenced Drosophila species, as well as in some other Dipterans. Twenty-three genes are associated with both an in vivo peak in DSX binding and an optimal DSX-binding sequence, and thus are almost certainly direct DSX targets. The association of these 23 genes with optimum DSX binding sites was used to examine the evolutionary changes occurring in DSX and its targets in insects.