ASICs Do Not Play a Role in Maintaining Hyperalgesia Induced by Repeated Intramuscular Acid Injections.
ASICs Do Not Play a Role in Maintaining Hyperalgesia Induced by Repeated Intramuscular Acid Injections.
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DOI：
10.1155/2012/817347
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发表时间：
2012
影响因子：
--
通讯作者：
Sluka KA
中科院分区：
文献类型：
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作者：
Gautam M;Benson CJ;Ranier JD;Light AR;Sluka KA
反复肌肉注射酸会产生依赖于ASICs激活的持久机械性痛敏。本研究调查了支配肌肉的感觉神经元的pH激活电流是否因反复注射酸而改变，以及阻断ASICs是否逆转了现有的痛觉过敏。在肌肉感觉神经元中，注射酸性生理盐水后，肌肉感觉神经元的平均酸诱发电流幅度和ASIC样电流的生物物理特性与注射中性pH生理盐水或未注射对照相比没有变化。此外，肌肉和爪部在第二次注射酸后机械敏感性的增加不受肌肉中ASICs(A-317567)的局部阻断的影响。作为对照，电子显微镜分析显示，注射酸后的胫神经未受损害。我们先前的研究表明，ASICs在反复注射酸引起的痛觉过敏的发展过程中起着重要作用。然而，目前的数据表明，ASICs不参与维持肌肉内反复注射酸的痛敏反应。
Repeated intramuscular acid injections produce long-lasting mechanical hyperalgesia that depends on activation of ASICs. The present study investigated if pH-activated currents in sensory neurons innervating muscle were altered in response to repeated acid injections, and if blockade of ASICs reverses existing hyperalgesia. In muscle sensory neurons, the mean acid-evoked current amplitudes and the biophysical properties of the ASIC-like currents were unchanged following acidic saline injections when compared to neutral pH saline injections or uninjected controls. Moreover, increased mechanical sensitivity of the muscle and paw after the second acid injection was unaffected by local blockade of ASICs (A-317567) in the muscle. As a control, electron microscopic analysis showed that the tibial nerve was undamaged after acid injections. Our previous studies demonstrated that ASICs are important in the development of hyperalgesia to repeated acid injections. However, the current data suggest that ASICs are not involved in maintaining hyperalgesia to repeated intramuscular acid injections.