The proteoglycan repertoire of lymphoid cells.
The proteoglycan repertoire of lymphoid cells.
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DOI：
10.1007/s10719-012-9427-9
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发表时间：
2012-10
影响因子：
3
通讯作者：
Uhlin-Hansen, Lars
中科院分区：
文献类型：
--
作者：
Fadnes, Bodil;Husebekk, Anne;Svineng, Gunbjorg;Rekdal, Oystein;Yanagishita, Masaki;Kolset, Svein O.;Uhlin-Hansen, Lars
在淋巴样细胞中对蛋白聚糖进行了有限程度的研究。在这项研究中，我们研究了蛋白聚糖在B细胞，CD 4 + T细胞，CD 8 + T细胞，自然杀伤细胞，以及在9个不同的细胞系建立淋巴恶性肿瘤患者的表达。丝甘肽是原代淋巴细胞在mRNA水平上表达的主要蛋白多糖。在原代淋巴细胞中，除了在CD 4 + T细胞和CD 8 + T细胞中检测到syndecan-4外，在mRNA水平上未检测到syndecan或glycipicans。所有淋巴样细胞系均表达丝甘肽mRNA，以及多配体蛋白聚糖和磷脂酰肌醇蛋白聚糖家族的一个或几个成员。此外，与原代淋巴细胞相比，在细胞系中发现蛋白聚糖的合成增加，以及在5个细胞系的细胞表面上存在硫酸乙酰肝素。Western blot分析显示sergylycin mRNA水平与sergylycin核心蛋白表达密切相关。我们的研究结果表明，sergylycin是一个主要的蛋白多糖在所有正常的淋巴细胞，这些细胞进行很少，或没有，蛋白多糖的细胞表面上。丝甘肽也是恶性淋巴细胞中的主要蛋白多糖，但这些细胞也表达一种或多种类型的细胞表面蛋白多糖。因此，淋巴样细胞的恶性转化可能伴随着蛋白聚糖合成的增加和细胞表面蛋白聚糖的表达。
Proteoglycans have been studied to a limited extent in lymphoid cells. In this study we have investigated the expression of proteoglycans in B-cells, CD4+ T-cells, CD8+ T-cells, natural killer cells, as well as in nine different cell lines established from patients with lymphoid malignancies. Serglycin was the major proteoglycan expressed at mRNA level by the primary lymphocytes. None of the syndecans or glycpicans was detected at mRNA level in the primary lymphocytes, except for syndecan-4 in CD4+ T-cells and CD8+ T-cells. All lymphoid cell lines expressed serglycin mRNA, as well as one or several members of the syndecan and glypican families. Further, increased synthesis of proteoglycans was found in the cell lines compared to the primary lymphocytes, as well as the presence of heparan sulfate on the cell surface of five of the cells lines. Western blot analysis showed a close correlation between serglycin mRNA level and expression of serglycin core protein. Our results show that serglycin is a major proteoglycan in all the normal lymphoid cells and that these cells carry little, or none, proteoglycans on the cell surface. Serglycin was also a major proteoglycan in the malignant lymphoid cells, but these also expressed one or more types of cell surface proteoglycans. Thus, malignant transformation of lymphoid cells may be followed by increased synthesis of proteoglycans and expression of cell surface proteoglycans.
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影响因子：
5.4
作者：
Chung, Jin-Sung;Bonkobara, Makoto;Tomihari, Mizuki;Cruz, Ponciano A., Jr.;Ariizumi, Kiyoshi
通讯作者：
Ariizumi, Kiyoshi
影响因子：
11.4
作者：
Niemann, C. U.;Kjeldsen, L.;Borregaard, N.
通讯作者：
Borregaard, N.
影响因子：
4.4
作者：
Duelli, Annette;Ronnberg, Elin;Pejler, Gunnar
通讯作者：
Pejler, Gunnar
影响因子：
32.4
作者：
Metkar, SS;Wang, BK;Froelich, CJ
通讯作者：
Froelich, CJ
影响因子：
4.8
作者：
Grujic, M;Braga, T;Åbrink, M
通讯作者：
Åbrink, M