From Vascular Corrosion Cast to Electrical Analog Model for the Study of Human Liver Hemodynamics and Perfusion
From Vascular Corrosion Cast to Electrical Analog Model for the Study of Human Liver Hemodynamics and Perfusion
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DOI：
10.1109/tbme.2010.2065229
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发表时间：
2011-01-01
影响因子：
4.6
通讯作者：
Segers, Patrick
中科院分区：
文献类型：
--
作者：
Debbaut, Charlotte;Monbaliu, Diethard;Segers, Patrick
由于静态冷藏的局限性和对扩大标准供体器官更好保存的需要，低温机器灌注（HMP）正在经历器官保存的复兴。对于肝脏，灌注方案仍然定义不清，并且窦状隙内皮细胞的损伤和不均匀灌注是关注的问题。在这项研究中，开发了一个人体肝脏血液循环的电模型，以揭示HMP过程中的内部压力和流量分布。通过结合血管腐蚀铸型、显微CT成像和图像处理获得了两个人肝脏的详细血管数据，用于建立电学模型。解剖数据可以在每棵树中测量多达5-6个血管代，并显示指数趋势线，用于预测更高代的数据。模拟的流量和压力与文献数据一致。该模型能够模拟压力驱动的HMP对肝脏血流动力学的影响，并再现观察结果，如肝动脉和门静脉之间的流量竞争。我们的模拟进一步表明，从纯生物力学（剪切应力）的角度来看，低压力的HMP不应该导致器官损伤，并且流体粘度对压力驱动的HMP中肝脏微循环所经历的剪切应力没有影响。
Hypothermic machine perfusion (HMP) is experiencing a revival in organ preservation due to the limitations of static cold storage and the need for better preservation of expanded criteria donor organs. For livers, perfusion protocols are still poorly defined, and damage of sinusoidal endothelial cells and heterogeneous perfusion are concerns. In this study, an electrical model of the human liver blood circulation is developed to enlighten internal pressure and flow distributions during HMP. Detailed vascular data on two human livers, obtained by combining vascular corrosion casting, micro-CT-imaging and image processing, were used to set up the electrical model. Anatomical data could be measured up to 5-6 vessel generations in each tree and showed exponential trend lines, used to predict data for higher generations. Simulated flow and pressure were in accordance with literature data. The model was able to simulate effects of pressure-driven HMP on liver hemodynamics and reproduced observations such as flow competition between the hepatic artery and portal vein. Our simulations further indicate that, from a pure biomechanical (shear stress) standpoint, HMP with low pressures should not result in organ damage, and that fluid viscosity has no effect on the shear stress experienced by the liver microcirculation in pressure-driven HMP.