Energy Regulated Nutritive and Antioxidant Properties during the Germination and Sprouting of Broccoli Sprouts (Brassica oleracea var. italica)
Energy Regulated Nutritive and Antioxidant Properties during the Germination and Sprouting of Broccoli Sprouts (Brassica oleracea var. italica)
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DOI：
10.1021/acs.jafc.8b00466
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发表时间：
2018-07-11
影响因子：
6.1
通讯作者：
Yang, Hongshun
中科院分区：
文献类型：
--
作者：
Chen, Lin;Tan, Glenna Jue Tong;Yang, Hongshun
通过外源ATP和DNP处理，研究了能量状态在青花菜种子萌发中的作用。随着腺苷酸的合成从38.82到142.69 mg. 100 g（-1）DW，营养成分（可溶性糖，蛋白质，色素和酚类化合物）和氨基酸增加发芽和早期发芽（第5天）。在能荷反馈抑制下，BoSnRK 2通路的元件下调超过2倍。在发芽结束时（第7天），能量消耗导致营养积累和抗氧化能力减缓或降低。外源ATP通过维持高水平的能量状态抑制BoSnRK 2途径，并进一步提高营养和抗氧化水平。它还防止了第7天的能量消耗。相反，DNP降低ATP含量（16.10-26.86%），并激活BoSnRK 2通路。对种子萌发、幼苗生长和次生代谢合成等耗能活动也有明显的抑制作用。
The role of energy status in germination and sprouting of broccoli seeds was investigated by exogenous ATP and DNP treatments. With the synthesis of adenylates from 38.82 to 142.69 mg.100 g(-1) DW, the nutritive components (soluble sugar, proteins, pigments, and phenolics) and AAs were increased during germination and early sprouting (day 5). Elements of the BoSnRK2 pathway were down-regulated by more than 2 fold under the energy charge feedback inhibition. At the end of sprouting (day 7), energy depletion resulted in slowdown or reduced nutritional accumulation and antioxidant capacities. Exogenous ATP depressed the BoSnRK2 pathway by maintaining the energy status at high levels and further promoted the nutrition and antioxidant levels. It also prevented the energy depletion at day 7. On the contrary, DNP reduced the ATP contents (16.10-26.86%) and activated the BoSnRK2 pathway. It also notably suppressed the energy-consuming activities including germination, sprouts growth, and secondary metabolic synthesis.