PHYLOGENY OF MYCOPLASMALIKE ORGANISMS (PHYTOPLASMAS) - A BASIS FOR THEIR CLASSIFICATION
PHYLOGENY OF MYCOPLASMALIKE ORGANISMS (PHYTOPLASMAS) - A BASIS FOR THEIR CLASSIFICATION
复制标题
DOI：
10.1128/jb.176.17.5244-5254.1994
复制
发表时间：
1994-09-01
影响因子：
3.2
通讯作者：
KINGSBURY, DT
中科院分区：
文献类型：
--
作者：
GUNDERSEN, DE;LEE, IM;KINGSBURY, DT
对46种无氧生物、19种类似支原体(MLO)(新名称为植原体)和几种相关细菌的16S rRNA基因序列进行了简约的系统发育分析，将MLO明确地归入Mollicuts纲的成员中，并揭示了MLO在厌氧体支系内形成了一个与无氧原体种同源的大的离散的单系支系。通过对来自30个不同的MLO的16S rRNA基因序列的简约分析，从代表几乎所有已知的不同的MLO组中，可以识别出五个主要的系统发育组，总共11个不同的亚支(单系群或TAR)。这些MLO亚类(罗马数字)和模式菌株分别是：I，马里兰州紫锥菊AY1；II，苹果增殖AP-A；III，花生丛枝PnWB；iv，加拿大桃X CX；v，水稻黄矮病Ryd；vi，木豆丛枝丛枝PPWB；vii，棕榈致死黄化LY；VIII，灰黄色；ix，三叶草增殖CP；x，榆黄EY；和xi，丝瓜丛枝LfWB。通过对最具代表性的MLO的核糖体蛋白L22基因序列的简约分析，得出了一致的系统发育，支持了MLO支系中亚支的命名及其系统发育位置。根据本研究所推断的系统发育，我们认为MLO应该在属的最低水平上进行分类，并且每个在系统发育上不同的MLO亚支至少应该代表这个新属下的一个不同的种。
A global phylogenetic analysis using parsimony of 16S rRNA gene sequences from 46 mollicutes, 19 mycoplasmalike organisms (MLOs) (new trivial name, phytoplasmas), and several related bacteria placed the MLOs definitively among the members of the class Mollicutes and revealed that MLOs form a large discrete monophyletic clade, paraphyletic to the Acholeplasma species, within the Anaeroplasma clade. Within the MLO clade resolved in the global mollicutes phylogeny and a comprehensive MLO phylogeny derived by parsimony analyses of 16S rRNA gene sequences from 30 diverse MLOs representative of nearly all known distinct MLO groups, five major phylogenetic groups with a total of 11 distinct subclades (monophyletic groups or tars) could be recognized. These MLO subclades (roman numerals) and designated type strains were as follows: i, Maryland aster yellows AY1; ii, apple proliferation AP-A; iii, peanut witches'-broom PnWB; iv, Canada peach X CX; v, rice yellow dwarf RYD; vi, pigeon pea witches'-broom PPWB; vii, palm lethal yellowing LY; viii, ash yellows AshY; ix, clover proliferation CP; x, elm yellows EY; and xi, loofah witches'-broom LfWB. The designations of subclades and their phylogenetic positions within the MLO clade were supported by a congruent phylogeny derived by parsimony analyses of ribosomal protein L22 gene sequences from most representative MLOs. On the basis of the phylogenies inferred in the present study, we propose that MLOs should be represented taxonomically at the minimal level of genus and that each phylogenetically distinct MLO subclade identified should represent at least a distinct species under this new genus.