Recognition of simian virus 40 T antigen synthesized during viral lytic cycle in monkey kidney cells expressing mouse H-2Kb- and H-2Db-transfected genes by SV40-specific cytotoxic T lymphocytes leads to the abrogation of virus lytic cycle.
Recognition of simian virus 40 T antigen synthesized during viral lytic cycle in monkey kidney cells expressing mouse H-2Kb- and H-2Db-transfected genes by SV40-specific cytotoxic T lymphocytes leads to the abrogation of virus lytic cycle.
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SV40 特异性细胞毒性 T 淋巴细胞对表达小鼠 H-2Kb 和 H-2Db 转染基因的猴肾细胞中病毒裂解周期期间合成的猿病毒 40 T 抗原进行识别，导致病毒裂解周期终止。
DOI：
10.1016/0042-6822(88)90409-6
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发表时间：
1988
期刊：
影响因子：
3.7
通讯作者：
Tevethia,SS
中科院分区：
文献类型：
--
作者：
Bates,MP;Jennings,SR;Tanaka,Y;Tevethia,MJ;Tevethia,SS
猴病毒40（SV 40）编码的肿瘤或T抗原定位于SV 40感染的容许猴细胞和SV 40转化的非容许细胞的细胞膜中。SV 40转化的小鼠细胞中的表面T抗原为细胞毒性T淋巴细胞（CTL）提供了靶标，所述细胞毒性T淋巴细胞（CTL）识别与鼠K/D I类H-2抗原相关的SV 40 T抗原。为了证明在SV 40感染的猴肾细胞（TC-7）中合成的SV 40 T抗原也可作为CTL的靶点，通过DNA转染在TC-7细胞中表达克隆的小鼠H β 2D和H β 2Kb基因，并经细胞分选后建立高表达H β 2Kb或H β 2Kb抗原的TC-7细胞系。SV 40感染的TC-7/H-2K和TC-7/H-2Db细胞对SV 40特异性H-2B限制性CTL的杀伤敏感。这些转染的SV 40感染的猴细胞对抗SV 40的混合培养CTL和SV 40特异性的H_2Db和H_2Kb限制性CTL克隆的敏感性依赖于SV 40 T抗原的合成和适当的H_2Kbor H_2Db限制性元件的表达。用CTL克隆处理SV 40感染的TC-7/H β 2D和TC-7/H β 2Kb，可阻断病毒的裂解周期，表明CTL在限制乳多空病毒感染天然宿主中可能起重要作用。
Simian virus 40 (SV40)-encoded tumor or T antigen localizes in the membranes in addition to the nucleus of SV40-infected permissive monkey cells and SV40-transformed nonpermissive cells. The surface T antigen in SV40-transformed mouse cells provides a target for the cytotoxic T lymphocytes (CTL) which recognize SV40 T antigen in association with murine K/D, class I H-2 antigens. In order to demonstrate that SV40 T antigen synthesized in SV40-infected permissive monkey kidney cells (TC-7) may also function as a target for CTL, cloned murine H2Dband H2Kbgenes were expressed in TC-7 cells by DNA transfection and TC-7 cell lines expressing high levels of either H2Kbor HDbantigens were established after cell sorting. SV40-infected TC-7/H2Kband TC-7/H2Dbcells became susceptible to lysis by SV40-specific H-2brestricted CTL. The susceptibility of these transfected SV40-infected monkey cells to anti-SV40 bulk culture CTL and SV40-specific H2Db- and H2Kb-restricted CTL clones depended upon the synthesis of SV40 T antigen and the expression of the appropriate H2Kbor H2Dbrestriction elements. Treatment of SV40-infected TC-7/H2Dband TC-7/H2KbWith CTL clones abrogated the virus lytic cycle indicating that CTL may play an important role in limiting papovavirus infection in the natural host.