ROLE OF SKIN SURFACE-LIPIDS IN UV-INDUCED EPIDERMAL-CELL CHANGES
ROLE OF SKIN SURFACE-LIPIDS IN UV-INDUCED EPIDERMAL-CELL CHANGES
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DOI：
10.1007/bf00372061
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发表时间：
1991-01-01
影响因子：
3
通讯作者：
PROTA, G
中科院分区：
文献类型：
--
作者：
PICARDO, M;ZOMPETTA, C;PROTA, G
紫外线照射能够在体外和体内影响皮肤表面脂质，特别是角鲨烯和胆固醇，产生活性脂质过氧化物。通过毛细管气相色谱法仔细评价皮肤脂质组分的光分解。紫外线诱导的脂质过氧化物对培养的人角质形成细胞和豚鼠耳片的影响与合成的脂质过氧化物，即。e.氢过氧化枯烯和13-氢过氧亚油酸酯。观察到对蛋白质合成和有丝分裂活性的时间和剂量依赖性影响。在细胞培养中，低浓度（0.05-5 μ g/ml）的过氧化角鲨烯和合成过氧化脂质刺激放射性标记的胸苷和苯丙氨酸的掺入，而较高浓度（> 10 μ g/ml）或较长时间的处理诱导细胞损伤。在豚鼠耳片中，过氧化脂质（5-50 μ g/ml）增加了氨基酸掺入和表皮色素细胞的数量;较高浓度（> 100 μ g/ml）导致表皮结构紊乱。结果表明，紫外线照射皮肤产生的脂质过氧化物从表面脂质，在体外，能够产生一些表皮细胞的变化。
Ultraviolet irradiation is capable of affecting skin surface lipids, especially squalene and cholesterol, both in vitro and in vivo, with generation of active lipoperoxides. The photodecomposition of the skin lipid component was carefully evaluated by capillary gas-chromatography. The effects of UV-induced lipoperoxides on human keratinocytes in culture and on guinea pig ear slices were compared with those of synthetic lipoperoxides, i. e. cumene hydroperoxide and 13-hydroperoxylinoleate. A time- and dose-dependent effect on protein synthesis and mitotic activity was observed. In cell culture low concentrations (0.05-5-mu-g/ml) of peroxidated squalene and synthetic lipoperoxides stimulated the incorporation of radiolabelled thymidine and phenylalanine, while higher concentrations (> 10-mu-g/ml), or longer periods of treatment, induced cellular damage. In guinea pig ear slices, the lipoperoxides (5-50-ug/ml) increased aminoacid incorporation and the number of epidermal pigment cells; higher concentrations (> 100-mu-g/ml) caused a derangement of epidermal structure. The results suggest that UV irradiation of skin generates lipoperoxides from the surface lipids which, in vitro, are capable of producing a number of changes in epidermal cells.