Thiolated Dendrimers as Multi-Point Binding Headgroups for DNA Immobilization on Gold
Thiolated Dendrimers as Multi-Point Binding Headgroups for DNA Immobilization on Gold
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DOI：
10.1021/la202444s
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发表时间：
2011-10-18
期刊：
影响因子：
3.9
通讯作者：
Norton, Michael L.
中科院分区：
文献类型：
--
作者：
Day, B. Scott;Fiegland, Larry R.;Norton, Michael L.
多硫醇化的DNA分子，可用于生产自组装单分子膜的单链DNA寡核苷酸在金基板上的合成进行了描述。第3代聚酰胺胺（PAMAM）树枝状聚合物与DNA低聚物缀合并用类似于30个保护的硫醇基团官能化。受保护的硫醇基团-硫代乙酸酯基团-允许树枝状体-DNA构建体在脱保护之前在缓冲溶液中储存至少2个月，而它们组装成功能层的能力没有任何可观察到的降低。使用这些多硫醇化DNA探针链形成的单层显示出与用单硫醇基团官能化的DNA组装的类似单层的靶捕获效率相当的靶捕获效率。使用树枝状聚合物头基的功能优势是在高温（95 ° C）下长时间暴露于缓冲溶液中时对探针链损失的抗性。
The synthesis of multithiolated DNA molecules that can be used to produce self-assembled monolayers of single-stranded DNA oligonucleotides on gold substrates is described. Generation 3 polyamidoamine (PAMAM) dendrimers were conjugated to DNA oligomers and functionalized with similar to 30 protected thiol groups. The protected thiol groups-thioacetate groups-allowed the dendrirner-DNA constructs to be stored in a buffer solution for at least 2 months before deprotection without any observable decrease in their ability to assemble into functional layers. The monolayers formed using these multithiolated DNA probe strands demonstrate target capture efficiencies comparable to those of analogous monolayers assembled with DNA functionalized with single thiol groups. A functional advantage of using dendrimer headgroups is the resistance to probe strand loss in prolonged exposure to buffer solutions at a high temperature (95 degrees C).