Typing of Staphylococcus aureus by PCR for DNA sequences flanked by transposon Tn916 target region and ribosomal binding site
Typing of Staphylococcus aureus by PCR for DNA sequences flanked by transposon Tn916 target region and ribosomal binding site
复制标题
DOI：
10.1128/jcm.34.6.1502-1505.1996
复制
发表时间：
1996-06-01
影响因子：
9.4
通讯作者：
Witte, W
中科院分区：
文献类型：
--
作者：
Cuny, C;Witte, W
多重耐药金黄色葡萄球菌在医院内和医院间的持续传播需要快速的分子分型系统。本研究通过 PCR 扩增转座子 Tn916 靶位点、核糖体结合位点和邻近核苷酸（靶标 916-Shine-Dalgarno PCR [tar 916-shida PCR]）两侧的 DNA 序列来描述金黄色葡萄球菌的指纹图谱。已知 PCR 的两个起始点随机分布在金黄色葡萄球菌染色体上。通过使用 SmaI 宏限制性模式作为参考方法，表明该 PCR 基因分型可区分金黄色葡萄球菌主要克隆群的菌株（噬菌体模式 29、+、94,96 和 95 的菌株以及 II 组和 III 组模式），并鉴定了德国医院中流行的 6 种流行性耐甲氧西林金黄色葡萄球菌菌株，所有调查的菌株包括对甲氧西林敏感的金黄色葡萄球菌进行分型，Tar 916-shida模式在流行性耐甲氧西林金黄色葡萄球菌在不同医院之间的传播过程中保持稳定。
The continuous intra- and interhospital spread of multiresistant Staphylococcus aureus demands a rapid molecular typing system. This study describes the fingerprinting of S. aureus by PCR amplification of DNA sequences flanked by the target site for transposon Tn916 and the ribosomal binding site and neighboring nucleotides (target 916-Shine-Dalgarno PCR [tar 916-shida PCR]). Both starting points for PCR are known to be randomly distributed on the S. aureus chromosome. By use of SmaI-macrorestriction patterns as the reference method it was shown that this PCR genotyping discriminates among strains of the major clonal groups of the species S. aureus (strains with phage patterns 29,+, 94,96, and 95 as well as group II and group III patterns) and identifies the six epidemic methicillin-resistant S. aureus strains prevalent in German hospitals, All of the investigated strains including methicillin-sensitive S. aureus were typeable, Tar 916-shida patterns are stable during the dissemination of epidemic methicillin-resistant S. aureus among different hospitals.