Polyadenine-Modulated DNA Conformation Monitored by Surface-Enhanced Raman Scattering (SERS) on Multibranched Gold Nanoparticles and Its Sensing Application
Polyadenine-Modulated DNA Conformation Monitored by Surface-Enhanced Raman Scattering (SERS) on Multibranched Gold Nanoparticles and Its Sensing Application
复制标题
多支链金纳米颗粒表面增强拉曼散射 (SERS) 监测聚腺嘌呤调节 DNA 构象及其传感应用
DOI：
10.1002/chem.201700883
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发表时间：
2017-07-12
影响因子：
4.3
通讯作者：
Ju, Huangxian
中科院分区：
文献类型：
--
作者：
Guo, Jingxing;Chen, Yunlong;Ju, Huangxian
这项工作提出了一种简便的方法来调节多分支金纳米粒子 (AuNP) 表面上的 DNA 构象从“躺下”构象到“站立”构象。这是通过调节连接到 DNA 序列的聚腺嘌呤 (polyA) 的长度和/或该序列与目标 DNA 的杂交来实现的，并且由于多支链金纳米粒子 (AuNSs) 作为表面增强拉曼散射 (SERS) 底物的优异性能以及拉曼报道分子和底物之间的距离变化，可以通过拉曼信号进行监测。可能的机制取决于多聚A对序列和尖端组件的排斥，也通过使用有限差分时域方法的理论模拟进行了探讨。利用这一策略，构建了一种基于构象转化的DNA@AuNS传感器，用于识别特定的寡核苷酸，该传感器已用于检测与严重急性呼吸综合征（SARS）相关的DNA序列。该策略产生了一种具有良好可扩展性的新型传感平台，可用于 DNA 分析，并为促进金属表面 DNA 构象的认知提供了强大的协议。
This work proposes a facile way to modulate the conformation of DNA from the "Lie-Down" to the "Stand-Up" conformation on the surface of multibranched gold nanoparticles (AuNPs). This is realized by regulating the length of polyadenine (polyA) linked to the DNA sequence and/or the hybridization of this sequence with the target DNA, and can be monitored by the Raman signal owing to the excellent performance of multibranched AuNPs (AuNSs) as a surface-enhanced Raman scattering (SERS) substrate and the distance change between the Raman reporter and the substrate. The probable mechanism, which depends on the repulsion of polyA from the sequence and the tip assembly, has also been probed through theoretical simulation using the finite difference time domain method. By virtue of this strategy, a conformation-transformation-based DNA@AuNS sensor is constructed for the identification of a specific oligonucleotide, which has been used for the detection of DNA sequences associated with Severe Acute Respiratory Syndrome (SARS). This strategy leads to a novel sensing platform with good extendibility for DNA analysis, and provides a powerful protocol for facilitating the cognition of DNA conformation on metal surfaces.