Tuning the surface charge of phospholipid bilayers inhibits insulin fibrilization.
Tuning the surface charge of phospholipid bilayers inhibits insulin fibrilization.
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DOI：
10.1016/j.colsurfb.2022.112904
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发表时间：
2022-12
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影响因子：
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The interactions between proteins and materials, in particular lipid bilayers, has been studied extensively for their relevance in diseases and for the formulation of protein-based therapeutics and vaccines. However, the precise rules by which material properties induce favorable or unfavorable structural states in biomolecules are incompletely understood, and as a result, the rational design of materials remains challenging. Here, we investigated the influence of lipid bilayers (in the form of small unilamellar vesicles) on the formation of insulin amyloid fibrils using a fibril-specific assay (thioflavin T), polyacrylamide gel electrophoresis, and circular dichroism spectroscopy. Lipid bilayers composed of equal mixtures of cationic and anionic lipids effectively inhibited fibril formation and stabilized insulin in its native conformation. However, other lipid bilayer compositions failed to inhibit fibril formation or even destabilized insulin, exacerbating fibrilization and/or non-amyloid aggregation. Our findings suggest that electrostatic interactions with lipid bilayers can play a critical role in stabilizing or destabilizing insulin, and preventing the conversion of insulin to its amyloidogenic, disease-associated state.
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影响因子：
2.9
作者：
Chi, Eva Y.;Ege, Canay;Lee, Ka Yee C.
通讯作者：
Lee, Ka Yee C.
DOI：
10.1016/j.bbapap.2010.04.001
发表时间：
2010-07
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Biancalana M;Koide S
通讯作者：
Koide S
影响因子：
4.2
作者：
Menard LM;Wood NB;Vigoreaux JO
通讯作者：
Vigoreaux JO
影响因子：
3
作者：
Groenning, Minna;Norrman, Mathias;Frokjaer, Sven
通讯作者：
Frokjaer, Sven
影响因子：
5.4
作者：
Chaparro Sosa, Andres F.;Black, Kenneth J.;Schwartz, Daniel K.
通讯作者：
Schwartz, Daniel K.