Solution-processed graphene films for electrochemical monitoring of extracellular nitric oxide released by breast cancer cells
Solution-processed graphene films for electrochemical monitoring of extracellular nitric oxide released by breast cancer cells
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DOI：
10.1088/2053-1583/ad1251
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发表时间：
2024-01-01
期刊：
影响因子：
5.5
通讯作者：
Ebrahimi，Aida
中科院分区：
文献类型：
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作者：
Butler，Derrick;Sankhe，Chinmay S.;Ebrahimi，Aida
一氧化氮（NO）在心血管功能、免疫反应和细胞间信号传导中起重要作用。然而，由于其寿命短，实时检测一氧化氮是具有挑战性的。本文采用旋转涂层和热板退火的简易制造方法，开发了一种基于纤维连接蛋白修饰的溶液处理石墨烯墨水的电化学传感器，用于NO检测。该传感器首先用NO供体精胺NONOate进行了电化学表征，其动态范围为10-1000 μM。光学显微镜证实，纤维连接蛋白功能化的石墨烯支持MDA-MB-231乳腺癌细胞的附着和生长。l -精氨酸可以刺激细胞外NO的产生。NO的产生导致粘附细胞的形态变化，这种变化在加入NO合酶拮抗剂n ω-硝基- l -精氨酸甲酯后是可逆的。使用纤维连接蛋白功能化石墨烯传感器进行实时安培测量，也可以确认NO的产生。虽然这项工作的重点是NO检测，但这个潜在的可扩展平台可以扩展到其他细胞类型，包括治疗方法和生物相容性涂层的高通量评估。
Nitric oxide (NO) plays an important role in cardiovascular function, immune response, and intercellular signaling. However, due to its short lifetime, real-time detection of NO is challenging. Herein, an electrochemical sensor based on fibronectin-modified, solution-processed graphene ink for NO detection is developed using a facile fabrication method involving spin-coating and hot-plate annealing. The sensor is first electrochemically characterized with a NO donor, spermine NONOate, exhibiting a dynamic range of 10–1000 μM. The fibronectin-functionalized graphene supports the attachment and growth of MDA-MB-231 breast cancer cells, as confirmed by optical microscopy. Extracellular NO production is stimulated using the amino acid L-arginine. NO production results in morphological changes to the adhered cells, which are reversible upon the addition of the NO synthase antagonist Nω-nitro-L-arginine methyl ester. The production of NO is also confirmed using real-time amperometric measurements with the fibronectin-functionalized graphene sensors. While this work focuses on NO detection, this potentially scalable platform could be extended to other cell types with envisioned applications including the high-throughput evaluation of therapeutics and biocompatible coatings.