Proteomic analysis of protein changes in plasma by balloon test occlusion
Proteomic analysis of protein changes in plasma by balloon test occlusion
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DOI：
10.1016/j.jocn.2019.12.005
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发表时间：
2019-12
影响因子：
2
通讯作者：
T. Ozaki;R. Muramatsu;Hajime Nakamura;M. Kinoshita;H. Kishima;T. Yamashita
中科院分区：
文献类型：
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作者：
T. Ozaki;R. Muramatsu;Hajime Nakamura;M. Kinoshita;H. Kishima;T. Yamashita
短暂性缺血提供了对脑中长期缺血的耐受性。在小鼠实验模型中，短暂性脑缺血改变了循环蛋白质的组成比例，这与神经保护有关;然而，缺乏人体证据。本文模拟颈动脉球囊闭塞试验（BTO），观察了人血浆中循环蛋白组成比的变化。我们收集了9名患者（5名男性和4名女性;平均年龄64.2岁;范围45至77岁）BTO前和48小时后的血液样本，并使用LC-MS/MS分析研究了蛋白质组中循环分子水平的变化。BTO后血液样本中富含亮氨酸的α-2-糖蛋白和血清淀粉样蛋白A-1增加，蛋白AMBP减少。脑中血流的瞬时变化改变了血浆中的分子表达。由于动物模型中血浆蛋白组成的改变与缺血耐受有关，因此BTO改变水平的蛋白质也可能参与人体抗缺血的神经保护作用。
Transient ischemia provides the tolerance against prolonged ischemia in the brain. In mouse experimental model, transient ischemia changes the composition ratio of circulating proteins, which associate with neuroprotection; however, the human evidence is lacking. Here we mimicked balloon test occlusion (BTO) of carotid artery as a transient ischemia and investigated the change of composition ratio of the circulating protein in the human plasma. We collected blood samples from nine patients (5 men and 4 women; mean age 64.2 years; range 45 to 77 years) before and 48 h after BTO and investigated the changes of circulating molecules level in the proteome using LC-MS/MS analysis. Leucine-rich alpha-2-glycoprotein and serum amyloid A-1 increased and protein AMBP decreased in the blood samples after BTO. Transient change of blood flow in the brain alters molecular expression in the plasma. Because the alteration of plasma protein composition is involved in ischemic tolerance in animal models, the proteins whose level was changed by BTO may be also involved in neuroprotection against ischemia in human.