Microalgal photosynthetic inhibition and mixotrophic growth in Post Hydrothermal Liquefaction Wastewater (PHW)
Microalgal photosynthetic inhibition and mixotrophic growth in Post Hydrothermal Liquefaction Wastewater (PHW)
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DOI：
10.1016/j.algal.2021.102548
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发表时间：
2021-12
期刊：
影响因子：
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通讯作者：
M. Stablein;Douglas H. Baracho;Jamison Watson;Jaqueline C. Silva;Yuanhui Zhang;A. T. Lombardi
中科院分区：
文献类型：
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作者：
M. Stablein;Douglas H. Baracho;Jamison Watson;Jaqueline C. Silva;Yuanhui Zhang;A. T. Lombardi
水热液化将湿有机生物质转化为可再生的生物原油，同时产生有毒废水（PHW），然而，富含营养物质和有机化合物。虽然已经报道了在稀释的PHW中抑制藻类，但是根本原因仍然不清楚。本研究首次探讨了PHW对4种淡水绿色微藻（Chlorolobion braunii、Chlorellasorokiniana、Chlorella vulgaris和Scenedesmus quadricauda）生长、光合自养功能和生物量特性的影响，并研究了4种PHW浓度（0、0.5、1和2%v/v）对4种微藻的生理响应。基于脉冲幅度荧光法（PAM），在光合作用中的一般抑制效应被揭示，表明减少光合自养活性。快速光变曲线（RLC）表明，不同的光合作用阶段受到影响，最大量子产额下降，而Gompertz人口增长模型突出的增长速度和滞后阶段的差异。生理调节通过降低细胞活力、增加细胞大小和改变色素轮廓来建议。sorokiniana基于其在PHW中有限的抑制作用和生物量产生而突出。减少光合性能与增加的生长速率相结合，肯定了兼养。这些研究结果增加了越来越多的文献的PHW和类似的废水的回收利用提供了新的见解，考虑藻类光生物学和潜在的抑制机制，通过兼养代谢提高生物量的生产。
Hydrothermal Liquefaction converts wet organic biomass into renewable biocrude oil and simultaneously generates a toxic wastewater (PHW) that is, however, rich in nutrients and organic compounds. While inhibition of algae in diluted PHW has been reported, the underlying reasons are still unclear. The present research explores, for the first time, the effects of PHW on the growth, photoautotrophic functions, and biomass characteristics of four freshwater green microalgae.Chlorolobion braunii,Chlorellasorokiniana,Chlorella vulgaris, and Scenedesmus quadricauda were exposed to four PHW concentrations (0, 0.5, 1, and 2%v/v) and their physiological responses comprehensively investigated. Based in pulse amplitude fluorometry (PAM), a general inhibitory effect in photosynthesis was revealed, indicating diminished photoautotrophic activity. Rapid light curve (RLC) showed that different photosynthetic stages were affected and maximum quantum yield decreased, while Gompertz modeling of population growth highlighted differences in both growth rates and lag phases. Physiological adjustment is suggested by reduced cell viability, increased cell size, and modification in pigment profiles.C. sorokinianastood out based on its limited inhibitory effects and biomass generation in PHW. The combination of diminished photosynthetic performance with increased growth rate affirmed mixotrophy. These findings add to a growing body of literature on the recycling of PHW and similar wastewaters by providing new insights into inhibition mechanisms considering algal photobiology and potential for enhanced biomass production through mixotrophic metabolism.