Neospora caninum-infected cattle develop parasite-specific CD4+ cytotoxic T lymphocytes.
Neospora caninum-infected cattle develop parasite-specific CD4+ cytotoxic T lymphocytes.
复制标题
感染犬新孢子虫的牛会产生寄生虫特异性 CD4 细胞毒性 T 淋巴细胞。
DOI：
10.1128/iai.71.6.3272-3279.2003
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发表时间：
2003
影响因子：
3.1
通讯作者：
Baszler,TimothyV
中科院分区：
文献类型：
--
作者：
Staska,LaurenM;McGuire,TravisC;Davies,ChristopherJ;Lewin,HarrisA;Baszler,TimothyV
感染犬新孢子虫的牛很容易经历经胎盘的寄生虫传播，可能是在母体寄生虫血症后，导致流产或出生先天性感染的小牛。细胞毒性T淋巴细胞（CTL）是抗弓形虫（Toxoplasma gondii）保护性免疫的重要介质。犬的在本研究中，N.用~（51）Cr释放细胞毒性试验鉴定了从两种主要组织相容性复合物不匹配的实验感染牛的外周血单个核细胞中扩增的犬特异性CTL。CD 4+和CD 8 + T淋巴细胞效应子亚群的富集和阻断表明，CD 4 +CTL杀伤N。犬感染的自体靶细胞，并且杀伤是通过穿孔素/颗粒酶途径介导的。检测和鉴定CTL对N.犬毒素在天然远交牛宿主中的应用将有助于免疫原的鉴定和免疫策略的设计，以诱导针对transplantalN的寄生虫特异性CTL。在牛中的犬传染。
Cattle infected withNeospora caninumreadily experience transplacental parasite transmission, presumably after maternal parasitemia, leading to abortion or birth of congenitally infected calves. Cytotoxic T lymphocytes (CTL) are important mediators of protective immunity againstToxoplasma gondii, an intracellular apicomplexan protozoan closely related toN. caninum. In this study,N. caninum-specific CTL expanded from peripheral blood mononuclear cells of two major histocompatibility complex-mismatched, experimentally infected cattle were identified by using a51Cr release cytotoxicity assay. Enrichment and blocking of CD4+- and CD8+-T-lymphocyte effector subsets indicated that CD4+CTL killedN. caninum-infected, autologous target cells and that killing was mediated through a perforin/granzyme pathway. Detection and characterization of CTL responses toN. caninumin the natural, outbred, bovine host will facilitate identification of immunogens and design of immunization strategies to induce parasite-specific CTL against transplacentalN. caninumtransmission in cattle.