Studies on in vitro synthesis and secretion of growth hormone and prolactin. II. Evidence against the existence of precursor molecules.
Studies on in vitro synthesis and secretion of growth hormone and prolactin. II. Evidence against the existence of precursor molecules.
复制标题
生长激素和催乳素体外合成与分泌的研究。
DOI：
10.1210/endo-94-1-104
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发表时间：
1974
期刊：
影响因子：
4.8
通讯作者：
J. Meldolesi
中科院分区：
文献类型：
--
作者：
A. Zanini;G. Giannattasio;J. Meldolesi
为了建立可能存在的激素前体，大鼠生长激素的生物合成。(GH)用~（14）C-L-亮氨酸脉冲标记垂体脑片5 min，然后在chase培养基中孵育40 min，用两种不同的凝胶电泳技术分析蛋白质（Ornstein和Davis的非解离凝胶系统和高分辨率SDS凝胶系统）和14 C-L-掺入率。亮氨酸和总蛋白以及GH和PRL和其他蛋白条带中Chase的比放射性的演变。没有动力学的激素标记相容的前体的存在时，得到的样品充分溶解与洗涤剂电泳前在任一系统。激素条带在脉冲结束时出现高度标记，其比放射性在追逐孵育期间没有显著变化。只有当匀浆不完全溶解时，
In order to establish the possible existence of hormone precursors, the biosynthesis of rat growth hormone. (GH) and prolactin (PRL) was investigated in pituitary slices pulselabeled for a short time (5 min) with 14C-Lleucine and then incubated in chase medium up to 40 min. Proteins were analyzed by two different techniques of gel electrophoresis (the nondissociating gel system of Ornstein and Davis and a high resolution SDS gel system) and the rate of incorporation of 14C-L-leucine and the evolution of the specific radioactivity with chase in total protein as well as in GH and PRL and in other protein bands were followed. No kinetics of hormone labeling compatible with the existence of precursors was ever obtained when samples were adequately solubilized with detergents prior to electrophoresis on either system. Hormone bands appeared highly labeled at the end of the pulse and their specific radioactivity did not change significantly during chase incubation. Only when homogenates were incompletely solubi...