Evaluation of agar gel immunodiffusion serology using caprine and ovine lentiviral antigens for detection of antibody to caprine arthritis-encephalitis virus.
Evaluation of agar gel immunodiffusion serology using caprine and ovine lentiviral antigens for detection of antibody to caprine arthritis-encephalitis virus.
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使用山羊和绵羊慢病毒抗原对琼脂凝胶免疫扩散血清学进行评估，以检测山羊关节炎脑炎病毒抗体。
DOI：
10.1128/jcm.32.1.243-245.1994
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发表时间：
1994
影响因子：
9.4
通讯作者：
Cheevers,WP
中科院分区：
文献类型：
--
作者：
KnowlesJr,DP;Evermann,JF;Shropshire,C;VanderSchalie,J;Bradway,D;Gezon,HM;Cheevers,WP
以山羊关节炎-脑炎病毒（CAEV）和绵羊进行性肺炎病毒（OPPV）为抗原源，研究了琼脂凝胶免疫扩散（AGID）试验检测CAEV抗体的敏感性。应用AGID法和免疫沉淀法对218份山羊血清标本进行CAEV抗体检测。与免疫沉淀法相比，CAEV AGID试验的灵敏度为0.91，OPPVAGID试验的灵敏度为0.56。用任一抗原进行的AGID试验具有100%特异性。OPPV-AGID试验检测CAEV抗体的敏感性较低，表明OPPV-AGID抗原在CAEV诊断和控制程序中的应用价值有限。
The sensitivity of the agar gel immunodiffusion (AGID) test for the detection of antibody to caprine arthritis-encephalitis virus (CAEV) was investigated with CAEV or ovine progressive pneumonia virus (OPPV) as the source of antigen. A total of 218 goat serum specimens were tested for anti-CAEV antibody by AGID and immunoprecipitation of [35S]methionine-labeled CAEV. In comparison with that of immunoprecipitation, the sensitivity of the CAEV AGID test was 0.91, and that of the OPPV AGID test was 0.56. The AGID test with either antigen was 100% specific. The lower sensitivity of the OPPV AGID test in detecting caprine antibody to CAEV indicates that OPPV antigen is of limited value for use in CAEV diagnosis and control programs.