Molecular characterization, expression patterns and polymorphism analysis of porcine Six1 gene
Molecular characterization, expression patterns and polymorphism analysis of porcine Six1 gene
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猪Six1基因的分子特征、表达模式及多态性分析
DOI：
10.1007/s11033-010-0403-9
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发表时间：
2011-04-01
影响因子：
2.8
通讯作者：
Xiong, Yuanzhu
中科院分区：
文献类型：
--
作者：
Wu, Wangjun;Ren, Zhuqing;Xiong, Yuanzhu
SIX1属于6个基因家族，在哺乳动物中包括6个成员(从SIX1到Six6)。已有研究证明SIX1同源结构域转录因子与肌肉发生有关。然而，SIX1在猪中的分子特性尚未见报道。在本研究中，我们分离并鉴定了猪SIX1基因的全长cDNA、基因组DNA和近端启动子序列。半定量RT-PCR结果证实，猪SIX1基因在骨骼肌中高效表达。实时荧光定量聚合酶链式反应结果表明，猪SIX1基因在胚胎期高度表达，梅山猪和约克夏猪的表达差异不显著。此外，SIX1在约克夏猪不同发育阶段的表达差异显著，从3日龄到180日龄表达呈上调趋势，在180日龄达到最高表达水平。此外，SIX1在不同肌肉中的表达数据表明，SIX1是一个快抽动肌肉高表达基因。建立了检测启动子C/T突变和内含子A/G突变的聚合酶链式反应-HindII-RFLP方法。同时，在眉山×约克夏F-2参考家系中对它们与经济性状的关联进行了评估。统计结果表明，猪SIX1的两个HindII多态与几个肉质性状显著相关(P<；0.05)。综上所述，这些研究表明，猪SIX1可能在骨骼肌生长和肉类品质中发挥重要作用。
Six1 belongs to the Six gene family that includes six members in mammals (from Six1 to Six6). It has been demonstrated that Six1 homeodomain transcription factor is implicated in myogenesis. However, the molecular characteristics of Six1 in pigs have not been reported. In this study, we isolated and characterized the porcine Six1 gene full-length cDNA, genomic DNA and proximal promoter sequence. Semi-quantitative RT-PCR results confirmed that porcine Six1 was highly expressed in skeletal muscle. Real-time quantitative PCR results showed that porcine Six1 was highly expressed in embryonic stage and no significant expression differences is observed between Meishan and Yorkshire pigs. Furthermore, Six1 expression was significantly different among different developmental stages in Yorkshire pigs and the expression trend was up-regulated from 3 days to 180 days, reaching its highest expression level at 180 days. In addition, the data of Six1 expression in different muscles demonstrated that Six1 was a fast-twitch muscle high expression gene. PCR-HindII-RFLP was established to detect a C/T mutation and an A/G mutation which were present in promoter and intron, respectively. Meanwhile, their associations with economic traits were assessed in Meishan x Yorkshire F-2 reference family. The statistical results revealed that the two HindII polymorphisms of porcine Six1 had significant associations with several meat quality traits (P < 0.05). Taken together, these studies suggest that porcine Six1 may play an important role in skeletal muscle growth and meat quality.