A self-renewal assay for cancer stem cells
A self-renewal assay for cancer stem cells
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DOI：
10.1007/s00280-005-0097-1
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发表时间：
2005-11-01
影响因子：
3
通讯作者：
Clarke, MF
中科院分区：
文献类型：
--
作者：
Clarke, MF
上皮来源的癌症是美国大多数癌症相关死亡的原因。不幸的是，尽管化疗和/或放疗有时可以缩小肿瘤，但上皮来源的转移性癌症基本上是不可治愈的。显然，需要新的方法来治疗这些疾病。虽然癌细胞系提供了宝贵的信息，但它们的生物学特性往往与新生癌细胞在关键方面不同。我们的实验室开发了一种新的小鼠模型，可以可靠地检测直接从患者肿瘤中分离的单个癌细胞。这将允许表征参与过程的关键信号传导途径，例如对新生肿瘤内的癌细胞形成肿瘤至关重要的自我更新。这些工具应该导致对驱动人类乳腺癌生长和侵袭的细胞和分子机制的新见解。
Cancers of epithelial origin are responsible for the majority of cancer-related deaths in the USA. Unfortunately, although chemotherapy and/or radiation therapy can sometimes shrink tumors, metastatic cancers of epithelial origin are essentially incurable. It is clear that new approaches are needed to treat these diseases. Although cancer cell lines provide invaluable information, their biological properties often differ in crucial ways from de novo cancer cells. Our laboratory has developed a novel mouse model that reliably permits individual cancer cells isolated directly from patients' tumors to be assayed. This will allow the characterization of crucial signaling pathways involved in processes such as self-renewal that are critical for tumor formation by the cancer cells within de novo tumors. These tools should lead to new insights into the cellular and molecular mechanisms that drive human breast cancer growth and invasion.