Revealing the Transcriptome Landscape of Mouse Spermatogonial Cells by Tiling Microarray
Revealing the Transcriptome Landscape of Mouse Spermatogonial Cells by Tiling Microarray
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DOI：
10.1007/978-1-61779-436-0_7
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发表时间：
2012-01-01
期刊：
影响因子：
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通讯作者：
Chan, Wai-Yee
中科院分区：
文献类型：
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作者：
Lee, Tin-Lap;Rennert, Owen M.;Chan, Wai-Yee
在过去的十年中，微阵列技术的出现，使男性生殖细胞发育的功能基因组研究，导致在确定基因的各种过程。常规基因表达微阵列的主要限制是由于基因探针设计而导致获得的结果有偏差。用于表达微阵列的基因探针通常由位于转录物3'端的少量探针表示。平铺微阵列通过在整个基因组上平铺的探针以无偏的方式询问基因组来消除这样的问题。这些阵列提供了更高的基因组分辨率，并允许鉴定新的转录本。为了揭示发育中的雄性生殖细胞基因组景观的复杂性，我们应用平铺芯片来评估精原细胞的转录组。超过50%的已知小鼠基因在睾丸发育期间表达。超过47%的成绩单是uncharacterized。结果表明，精原细胞中的转录机制比以前设想的要复杂。
In the past decade, the advent of microarray technologies has allowed functional genomic studies of male germ cell development, resulting in the identification of genes governing various processes. A major limitation with conventional gene expression microarray is that results obtained are biased due to gene probe design. The gene probes for expression microarrays are usually represented by a small number of probes located at the 3' end of a transcript. Tiling microarrays eliminate such issue by interrogating the genome in an unbiased fashion through probes tiled across the entire genome. These arrays provide higher genomic resolution and allow the identification of novel transcripts. To reveal the complexity of the genomic landscape of developing male germ cells, we applied tiling microarray to evaluate the transcriptome in spermatogonial cells. Over 50% of all known mouse genes are expressed during testicular development. More than 47% of the transcripts are uncharacterized. The results suggested that the transcription machinery in spermaotogonial cells is more complex than previously envisioned.